miRNA: 3'- aUGCGCCUCgaGuaCcUGCGCGAGCU- -5'
miRNA: 3'- aUGCGCcUCgAGUACcUGCGCGa---GCU- -5'
miRNA: 3'- aUGCG-CCUCGAGuACC--UGC-GCGAgcu -5'
miRNA: 3'- aUGCGCCUCgAGUa---CCUGCGCGa---GCU- -5'
miRNA: 3'- augCGCCUcgAGUACCUGCGCGAgCU- -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29529 | 5' | -58.1 | NC_006151.1 | + | 138144 | 0.79 | 0.188849 |
|
Target: 5'- gACGCGGAGgaCcgGcGCGCGCUCGAg -3' miRNA: 3'- aUGCGCCUCgaGuaCcUGCGCGAGCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 138724 | 0.66 | 0.811487 |
|
Target: 5'- cGCGCGcGGgUCGUGuACGCGCgccgCGAc -3' miRNA: 3'- aUGCGCcUCgAGUACcUGCGCGa---GCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 139203 | 0.66 | 0.793762 |
|
Target: 5'- gGCGCuGGAGCUCcUGGcgGCGaCGCUg-- -3' miRNA: 3'- aUGCG-CCUCGAGuACC--UGC-GCGAgcu -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 139647 | 0.71 | 0.507101 |
|
Target: 5'- gGCGCGGGGgUCGcggcGGGCGCGCcgccCGAc -3' miRNA: 3'- aUGCGCCUCgAGUa---CCUGCGCGa---GCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 140461 | 0.72 | 0.432735 |
|
Target: 5'- --gGCGGGa-UCAUGGACGCGCUgGAc -3' miRNA: 3'- augCGCCUcgAGUACCUGCGCGAgCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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