miRNA: 3'- augCGCC-UCGAGUACC--UGC-GCGAGcu -5'
miRNA: 3'- aUGcGCCUCGa--GUaCCUG-----CGCGAGCU- -5'
miRNA: 3'- aUGCGCCUCGa------GUACCUG--------CGCGaGCU- -5'
miRNA: 3'- aUGCGCCUCGaguac-CUGCgCGAGCU- -5'
miRNA: 3'- aUGCGCCUCGAGUa-------CCUG--CGCGaGCU- -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29529 | 5' | -58.1 | NC_006151.1 | + | 4486 | 0.7 | 0.566239 |
|
Target: 5'- --aGCGGcGGCUCAUGGccACGgCGCUCa- -3' miRNA: 3'- augCGCC-UCGAGUACC--UGC-GCGAGcu -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 4245 | 0.67 | 0.7178 |
|
Target: 5'- aGC-CGGAGCgggCA-GGGCagcagGCGCUCGAg -3' miRNA: 3'- aUGcGCCUCGa--GUaCCUG-----CGCGAGCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 3457 | 0.68 | 0.677787 |
|
Target: 5'- gGCGCGGGGUgcucgggCAUGGGCccgagcggGCGC-CGGa -3' miRNA: 3'- aUGCGCCUCGa------GUACCUG--------CGCGaGCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 3137 | 0.7 | 0.556235 |
|
Target: 5'- gGCGCGGGGCgcccucGGCGgGCUCGGc -3' miRNA: 3'- aUGCGCCUCGaguac-CUGCgCGAGCU- -5' |
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| 29529 | 5' | -58.1 | NC_006151.1 | + | 2779 | 0.69 | 0.60665 |
|
Target: 5'- cGCGCGGAGCUCGcggcacccGGGCcaGCGCaCGGc -3' miRNA: 3'- aUGCGCCUCGAGUa-------CCUG--CGCGaGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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