miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
29536 3' -56.7 NC_006151.1 + 53342 0.66 0.854964
Target:  5'- cACGGGGCGCGCGcGcgccgcguacUGcGUCgUGUCGu -3'
miRNA:   3'- -UGCCCUGCGCGCaC----------ACaCAG-ACAGUu -5'
29536 3' -56.7 NC_006151.1 + 62381 0.66 0.838886
Target:  5'- uCGGGACGCGCGccguccUGgcgGUcCUGUCGc -3'
miRNA:   3'- uGCCCUGCGCGC------ACacaCA-GACAGUu -5'
29536 3' -56.7 NC_006151.1 + 75860 0.67 0.813361
Target:  5'- gACGGGGCGCcccuGCGUGUccgcGUGg--GUCAu -3'
miRNA:   3'- -UGCCCUGCG----CGCACA----CACagaCAGUu -5'
29536 3' -56.7 NC_006151.1 + 141752 0.7 0.668126
Target:  5'- cGCGGGGCGCGCGccccgGUGgcgucgccgGuUCUGUCc- -3'
miRNA:   3'- -UGCCCUGCGCGCa----CACa--------C-AGACAGuu -5'
29536 3' -56.7 NC_006151.1 + 31435 0.76 0.331435
Target:  5'- gACGGGgugcGCGCGCGUGUGUGUaUGUg-- -3'
miRNA:   3'- -UGCCC----UGCGCGCACACACAgACAguu -5'
29536 3' -56.7 NC_006151.1 + 8491 0.76 0.321745
Target:  5'- gACGGGGCGgGCGgggagggugucgucUGUGUGUCUGUg-- -3'
miRNA:   3'- -UGCCCUGCgCGC--------------ACACACAGACAguu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.