Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29536 | 3' | -56.7 | NC_006151.1 | + | 53342 | 0.66 | 0.854964 |
Target: 5'- cACGGGGCGCGCGcGcgccgcguacUGcGUCgUGUCGu -3' miRNA: 3'- -UGCCCUGCGCGCaC----------ACaCAG-ACAGUu -5' |
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29536 | 3' | -56.7 | NC_006151.1 | + | 62381 | 0.66 | 0.838886 |
Target: 5'- uCGGGACGCGCGccguccUGgcgGUcCUGUCGc -3' miRNA: 3'- uGCCCUGCGCGC------ACacaCA-GACAGUu -5' |
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29536 | 3' | -56.7 | NC_006151.1 | + | 75860 | 0.67 | 0.813361 |
Target: 5'- gACGGGGCGCcccuGCGUGUccgcGUGg--GUCAu -3' miRNA: 3'- -UGCCCUGCG----CGCACA----CACagaCAGUu -5' |
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29536 | 3' | -56.7 | NC_006151.1 | + | 141752 | 0.7 | 0.668126 |
Target: 5'- cGCGGGGCGCGCGccccgGUGgcgucgccgGuUCUGUCc- -3' miRNA: 3'- -UGCCCUGCGCGCa----CACa--------C-AGACAGuu -5' |
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29536 | 3' | -56.7 | NC_006151.1 | + | 31435 | 0.76 | 0.331435 |
Target: 5'- gACGGGgugcGCGCGCGUGUGUGUaUGUg-- -3' miRNA: 3'- -UGCCC----UGCGCGCACACACAgACAguu -5' |
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29536 | 3' | -56.7 | NC_006151.1 | + | 8491 | 0.76 | 0.321745 |
Target: 5'- gACGGGGCGgGCGgggagggugucgucUGUGUGUCUGUg-- -3' miRNA: 3'- -UGCCCUGCgCGC--------------ACACACAGACAguu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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