Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29540 | 3' | -51.8 | NC_006151.1 | + | 39833 | 0.66 | 0.985201 |
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Target: 5'- -gUCUUCGuc-UCCUGCgGCcgccgggGCCUCGc -3' miRNA: 3'- gaAGAAGUucaAGGACGgCG-------UGGAGC- -5' |
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| 29540 | 3' | -51.8 | NC_006151.1 | + | 66541 | 0.66 | 0.983516 |
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Target: 5'- -cUCUUCGGGggCgCgcgcgGCCGCGCCg-- -3' miRNA: 3'- gaAGAAGUUCaaG-Ga----CGGCGUGGagc -5' |
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| 29540 | 3' | -51.8 | NC_006151.1 | + | 123467 | 0.66 | 0.980831 |
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Target: 5'- --aCUUUGcGGUggagaacgugggccUCCUGCCGCACCUg- -3' miRNA: 3'- gaaGAAGU-UCA--------------AGGACGGCGUGGAgc -5' |
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| 29540 | 3' | -51.8 | NC_006151.1 | + | 131439 | 0.66 | 0.978314 |
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Target: 5'- -cUCgUCGGGcggcgggcggaagUCCgggcGCCGCACCUCGc -3' miRNA: 3'- gaAGaAGUUCa------------AGGa---CGGCGUGGAGC- -5' |
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| 29540 | 3' | -51.8 | NC_006151.1 | + | 121255 | 0.66 | 0.976842 |
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Target: 5'- -----aCGAGUUCCUggcgGCCGCGCUggCGg -3' miRNA: 3'- gaagaaGUUCAAGGA----CGGCGUGGa-GC- -5' |
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| 29540 | 3' | -51.8 | NC_006151.1 | + | 84567 | 0.67 | 0.974228 |
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Target: 5'- gCUUCagCGAGggCCUcGaCgCGCGCCUCGc -3' miRNA: 3'- -GAAGaaGUUCaaGGA-C-G-GCGUGGAGC- -5' |
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| 29540 | 3' | -51.8 | NC_006151.1 | + | 138497 | 0.67 | 0.961619 |
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Target: 5'- -cUCggCGGGcgCCcGCUGCACCUCc -3' miRNA: 3'- gaAGaaGUUCaaGGaCGGCGUGGAGc -5' |
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| 29540 | 3' | -51.8 | NC_006151.1 | + | 49412 | 0.7 | 0.906951 |
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Target: 5'- --cCUUCAGGa-CCUGcCCGCACUUUGg -3' miRNA: 3'- gaaGAAGUUCaaGGAC-GGCGUGGAGC- -5' |
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| 29540 | 3' | -51.8 | NC_006151.1 | + | 55748 | 0.7 | 0.900459 |
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Target: 5'- aCUUCUUCuccaUCCUGCgCGUgcgccACCUCGg -3' miRNA: 3'- -GAAGAAGuucaAGGACG-GCG-----UGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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