miRNA: 3'- aGGCGGa--GGUaCAGGcugUGCAGGUG- -5'
miRNA: 3'- aGGCGGAGGuacaGGCuGUGCaGGUG- -5'
miRNA: 3'- aGGCGGAGG---UAC---AGGCUGUGCA------GGUG- -5'
miRNA: 3'- -AGGC--GGAGGUacaGGCUG----UGCAGGUG- -5'
miRNA: 3'- -AGGC---GGAGGUACaGGCuGUGCAGGUG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29564 | 3' | -57.4 | NC_006151.1 | + | 135193 | 0.72 | 0.539895 |
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Target: 5'- gCCGCCgggCCA-GUCCca-GCGUCCGCu -3' miRNA: 3'- aGGCGGa--GGUaCAGGcugUGCAGGUG- -5' |
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| 29564 | 3' | -57.4 | NC_006151.1 | + | 69508 | 0.72 | 0.530088 |
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Target: 5'- gCCGCCUCCuccgCCGcCGCG-CCGCg -3' miRNA: 3'- aGGCGGAGGuacaGGCuGUGCaGGUG- -5' |
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| 29564 | 3' | -57.4 | NC_006151.1 | + | 84928 | 0.72 | 0.520347 |
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Target: 5'- gCCGCCUCCggcGUGcucUUCGGCACGUgguacgCCACg -3' miRNA: 3'- aGGCGGAGG---UAC---AGGCUGUGCA------GGUG- -5' |
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| 29564 | 3' | -57.4 | NC_006151.1 | + | 37374 | 0.72 | 0.510677 |
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Target: 5'- cUCCGacCCUCCGg--CCGACggccGCGUCCGCu -3' miRNA: 3'- -AGGC--GGAGGUacaGGCUG----UGCAGGUG- -5' |
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| 29564 | 3' | -57.4 | NC_006151.1 | + | 82968 | 0.75 | 0.376767 |
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Target: 5'- cUCCGucaCCUCCAUG-CCG-CGCGUCUGCg -3' miRNA: 3'- -AGGC---GGAGGUACaGGCuGUGCAGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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