Results 121 - 129 of 129 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29567 | 3' | -59.9 | NC_006151.1 | + | 15333 | 0.67 | 0.656859 |
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Target: 5'- aCCCGCGCgucCUCUCCCcGCCggUCAUCGa- -3' miRNA: 3'- -GGGUGUGa--GAGAGGG-CGG--GGUGGUgu -5' |
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| 29567 | 3' | -59.9 | NC_006151.1 | + | 72031 | 0.68 | 0.646916 |
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Target: 5'- cCCCGCGCggg----CGCCCCGCCGCGc -3' miRNA: 3'- -GGGUGUGagagaggGCGGGGUGGUGU- -5' |
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| 29567 | 3' | -59.9 | NC_006151.1 | + | 32621 | 0.68 | 0.646916 |
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Target: 5'- gCCGCGC-CUCUCCCuCCCgcuuccccccuCGCCGCc -3' miRNA: 3'- gGGUGUGaGAGAGGGcGGG-----------GUGGUGu -5' |
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| 29567 | 3' | -59.9 | NC_006151.1 | + | 85682 | 0.68 | 0.646916 |
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Target: 5'- aCCUGCGCUUcCUgCUGgCCCACUACGu -3' miRNA: 3'- -GGGUGUGAGaGAgGGCgGGGUGGUGU- -5' |
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| 29567 | 3' | -59.9 | NC_006151.1 | + | 56808 | 0.68 | 0.646916 |
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Target: 5'- cCCCucuCUCgCUCCC-CCCCgACCGCGg -3' miRNA: 3'- -GGGuguGAGaGAGGGcGGGG-UGGUGU- -5' |
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| 29567 | 3' | -59.9 | NC_006151.1 | + | 109820 | 0.68 | 0.636961 |
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Target: 5'- cCCCGCGCUC---CCCGCuggcggaccCCCACCAg- -3' miRNA: 3'- -GGGUGUGAGagaGGGCG---------GGGUGGUgu -5' |
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| 29567 | 3' | -59.9 | NC_006151.1 | + | 30539 | 0.68 | 0.636961 |
|
Target: 5'- cCCCGCucccccCUCUCgUUCCGCgCCGCaCGCGc -3' miRNA: 3'- -GGGUGu-----GAGAG-AGGGCGgGGUG-GUGU- -5' |
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| 29567 | 3' | -59.9 | NC_006151.1 | + | 8293 | 0.68 | 0.636961 |
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Target: 5'- cCCCGCGCgggCgccgCgUCCCGCCCCgaGCCc-- -3' miRNA: 3'- -GGGUGUGa--Ga---G-AGGGCGGGG--UGGugu -5' |
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| 29567 | 3' | -59.9 | NC_006151.1 | + | 66156 | 0.69 | 0.557779 |
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Target: 5'- gUgGCGCUCUacaUCCCGCCgggccuguuuggCCGCCAUAa -3' miRNA: 3'- gGgUGUGAGAg--AGGGCGG------------GGUGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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