Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29578 | 5' | -56.4 | NC_006151.1 | + | 139556 | 0.69 | 0.726135 |
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Target: 5'- aGCGaGGAGCGGCGCCCgguccgcgagcgCCUGG-GCu -3' miRNA: 3'- cUGCaUCUUGUCGUGGG------------GGACCaCGu -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 90733 | 0.69 | 0.716116 |
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Target: 5'- cGACgGUGGcGGCGGCGCCCUCgGGgGCGg -3' miRNA: 3'- -CUG-CAUC-UUGUCGUGGGGGaCCaCGU- -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 114963 | 0.71 | 0.603085 |
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Target: 5'- uGACGUAGGACAcGCGCUCCUUGacgcucgGCAc -3' miRNA: 3'- -CUGCAUCUUGU-CGUGGGGGACca-----CGU- -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 141848 | 0.72 | 0.521835 |
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Target: 5'- cGACGcgaGGGGCucgcgccGCGCCCUCUGGUGCc -3' miRNA: 3'- -CUGCa--UCUUGu------CGUGGGGGACCACGu -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 2981 | 0.77 | 0.298843 |
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Target: 5'- cGCGccgAGAGCagAGCACCCCCUGG-GCGu -3' miRNA: 3'- cUGCa--UCUUG--UCGUGGGGGACCaCGU- -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 20617 | 0.83 | 0.124202 |
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Target: 5'- cGGCGUGGggUggugggGGCGCCCCCUGGgcgGCAc -3' miRNA: 3'- -CUGCAUCuuG------UCGUGGGGGACCa--CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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