Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29578 | 5' | -56.4 | NC_006151.1 | + | 121320 | 0.66 | 0.853437 |
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Target: 5'- aGCGUGGAgGCGGcCGCCgagCUGGUGCGc -3' miRNA: 3'- cUGCAUCU-UGUC-GUGGgg-GACCACGU- -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 127476 | 0.66 | 0.876241 |
|
Target: 5'- cGGCGcGGAACgAGCcgcggACCCCgcgccggaagCUGGUGCGc -3' miRNA: 3'- -CUGCaUCUUG-UCG-----UGGGG----------GACCACGU- -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 131907 | 0.66 | 0.868855 |
|
Target: 5'- cGACG-GGGGCGGCagcaggGCCUCCgcGGUGCu -3' miRNA: 3'- -CUGCaUCUUGUCG------UGGGGGa-CCACGu -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 139556 | 0.69 | 0.726135 |
|
Target: 5'- aGCGaGGAGCGGCGCCCgguccgcgagcgCCUGG-GCu -3' miRNA: 3'- cUGCaUCUUGUCGUGGG------------GGACCaCGu -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 141848 | 0.72 | 0.521835 |
|
Target: 5'- cGACGcgaGGGGCucgcgccGCGCCCUCUGGUGCc -3' miRNA: 3'- -CUGCa--UCUUGu------CGUGGGGGACCACGu -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 142009 | 0.68 | 0.784173 |
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Target: 5'- cGGCGUGGGGCggguguGGCACCa-CgugGGUGCAu -3' miRNA: 3'- -CUGCAUCUUG------UCGUGGggGa--CCACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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