Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29578 | 5' | -56.4 | NC_006151.1 | + | 81631 | 0.66 | 0.845418 |
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Target: 5'- cGGCGaAGAAgAGCGCCUgCUGGU-CGa -3' miRNA: 3'- -CUGCaUCUUgUCGUGGGgGACCAcGU- -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 2672 | 0.66 | 0.845418 |
|
Target: 5'- uGGCGgcagAGGcgcaGCGGCucgGCCCCggGGUGCAg -3' miRNA: 3'- -CUGCa---UCU----UGUCG---UGGGGgaCCACGU- -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 50020 | 0.67 | 0.8372 |
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Target: 5'- cGCGgcccGcGCGGCGCCCCCgGGcGCGg -3' miRNA: 3'- cUGCau--CuUGUCGUGGGGGaCCaCGU- -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 97487 | 0.67 | 0.8372 |
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Target: 5'- cACGUGGAcgcGCAGCGCCUCgCggcGGUgGCGc -3' miRNA: 3'- cUGCAUCU---UGUCGUGGGG-Ga--CCA-CGU- -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 18298 | 0.67 | 0.836368 |
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Target: 5'- aGACGUcgaagccGGAGCggGGCGCCUCCUcGGgGCGc -3' miRNA: 3'- -CUGCA-------UCUUG--UCGUGGGGGA-CCaCGU- -5' |
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| 29578 | 5' | -56.4 | NC_006151.1 | + | 20617 | 0.83 | 0.124202 |
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Target: 5'- cGGCGUGGggUggugggGGCGCCCCCUGGgcgGCAc -3' miRNA: 3'- -CUGCAUCuuG------UCGUGGGGGACCa--CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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