Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2958 | 3' | -50.8 | NC_001493.1 | + | 85732 | 0.66 | 0.98875 |
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Target: 5'- aAUCAAUUGGGuucaccaacgaaGGCaUCACGGcguUCCAGu -3' miRNA: 3'- gUAGUUAGCCC------------UCGaAGUGCU---AGGUU- -5' |
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| 2958 | 3' | -50.8 | NC_001493.1 | + | 96137 | 0.66 | 0.987186 |
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Target: 5'- aAUCGG-CGGGAGUgacCACG-UCCAGg -3' miRNA: 3'- gUAGUUaGCCCUCGaa-GUGCuAGGUU- -5' |
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| 2958 | 3' | -50.8 | NC_001493.1 | + | 80125 | 0.66 | 0.985459 |
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Target: 5'- uUAUCGAUCGGGAGU---AUGAccgCCAAc -3' miRNA: 3'- -GUAGUUAGCCCUCGaagUGCUa--GGUU- -5' |
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| 2958 | 3' | -50.8 | NC_001493.1 | + | 62067 | 0.67 | 0.979208 |
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Target: 5'- uCGUCGcccGUUGGGAuaGCUgagcCGCGGUCCGu -3' miRNA: 3'- -GUAGU---UAGCCCU--CGAa---GUGCUAGGUu -5' |
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| 2958 | 3' | -50.8 | NC_001493.1 | + | 55624 | 0.67 | 0.976736 |
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Target: 5'- --gCGAUCGGG-GCUcugaUCGCGAUCa-- -3' miRNA: 3'- guaGUUAGCCCuCGA----AGUGCUAGguu -5' |
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| 2958 | 3' | -50.8 | NC_001493.1 | + | 130191 | 0.68 | 0.947886 |
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Target: 5'- aCAUCAuuGUCGGGGGCgaguggaugggCGCGGgugCCAc -3' miRNA: 3'- -GUAGU--UAGCCCUCGaa---------GUGCUa--GGUu -5' |
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| 2958 | 3' | -50.8 | NC_001493.1 | + | 14636 | 0.68 | 0.947886 |
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Target: 5'- aCAUCAuuGUCGGGGGCgaguggaugggCGCGGgugCCAc -3' miRNA: 3'- -GUAGU--UAGCCCUCGaa---------GUGCUa--GGUu -5' |
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| 2958 | 3' | -50.8 | NC_001493.1 | + | 64076 | 0.71 | 0.875993 |
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Target: 5'- uGUCGGUCGGGGGC---GCGuUCCGAg -3' miRNA: 3'- gUAGUUAGCCCUCGaagUGCuAGGUU- -5' |
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| 2958 | 3' | -50.8 | NC_001493.1 | + | 83032 | 1.06 | 0.010381 |
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Target: 5'- cCAUCAAUCGGGAGCUUCACGAUCCAAu -3' miRNA: 3'- -GUAGUUAGCCCUCGAAGUGCUAGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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