Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29594 | 5' | -54.1 | NC_006151.1 | + | 112412 | 0.66 | 0.915362 |
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Target: 5'- aUCgccgcgCGcAGAUCGAccGCCGCGCCGc -3' miRNA: 3'- aAGaa----GC-UCUAGUUcaCGGCGCGGUu -5' |
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| 29594 | 5' | -54.1 | NC_006151.1 | + | 92121 | 0.66 | 0.902714 |
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Target: 5'- aUCUUCGGGAacggccgcaUCGcGcGCgGCGCCAAg -3' miRNA: 3'- aAGAAGCUCU---------AGUuCaCGgCGCGGUU- -5' |
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| 29594 | 5' | -54.1 | NC_006151.1 | + | 99580 | 0.67 | 0.896008 |
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Target: 5'- -gCUgcgCGAGGUggcggcggcCGAGcGCCGCGCCGGg -3' miRNA: 3'- aaGAa--GCUCUA---------GUUCaCGGCGCGGUU- -5' |
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| 29594 | 5' | -54.1 | NC_006151.1 | + | 109290 | 0.68 | 0.858814 |
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Target: 5'- -cCUcCGAGAUCGAGcgGCC-CGCCGc -3' miRNA: 3'- aaGAaGCUCUAGUUCa-CGGcGCGGUu -5' |
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| 29594 | 5' | -54.1 | NC_006151.1 | + | 5736 | 0.68 | 0.850681 |
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Target: 5'- ----cCGGGGUcCGAG-GCCGCGCCGc -3' miRNA: 3'- aagaaGCUCUA-GUUCaCGGCGCGGUu -5' |
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| 29594 | 5' | -54.1 | NC_006151.1 | + | 135278 | 0.68 | 0.850681 |
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Target: 5'- cUCgaCGAGGUCGuccGUGuCCGCGUCGAc -3' miRNA: 3'- aAGaaGCUCUAGUu--CAC-GGCGCGGUU- -5' |
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| 29594 | 5' | -54.1 | NC_006151.1 | + | 40019 | 0.68 | 0.833776 |
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Target: 5'- -cCUUCGAGAcCAGcGU-CCGCGCCGc -3' miRNA: 3'- aaGAAGCUCUaGUU-CAcGGCGCGGUu -5' |
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| 29594 | 5' | -54.1 | NC_006151.1 | + | 57462 | 0.68 | 0.833776 |
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Target: 5'- ---gUCGGc--CAGGUGCCGCGCCGc -3' miRNA: 3'- aagaAGCUcuaGUUCACGGCGCGGUu -5' |
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| 29594 | 5' | -54.1 | NC_006151.1 | + | 66541 | 0.71 | 0.665639 |
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Target: 5'- cUCUUCGGGGgcgCGcGcgGCCGCGCCGc -3' miRNA: 3'- aAGAAGCUCUa--GUuCa-CGGCGCGGUu -5' |
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| 29594 | 5' | -54.1 | NC_006151.1 | + | 138056 | 0.72 | 0.612306 |
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Target: 5'- cUUCUUCGAGcgCAAG-GCgGCGCgGAa -3' miRNA: 3'- -AAGAAGCUCuaGUUCaCGgCGCGgUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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