Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29597 | 5' | -53.6 | NC_006151.1 | + | 139173 | 0.66 | 0.957084 |
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Target: 5'- cCUcGUGUGGAucuuuguGCUGgcGGUCGGGGc -3' miRNA: 3'- -GAcUACACCUu------CGACaaCCGGUCCUc -5' |
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| 29597 | 5' | -53.6 | NC_006151.1 | + | 17130 | 0.66 | 0.953085 |
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Target: 5'- -cGGUGcugcgGGAGGC----GGCCAGGAGc -3' miRNA: 3'- gaCUACa----CCUUCGacaaCCGGUCCUC- -5' |
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| 29597 | 5' | -53.6 | NC_006151.1 | + | 88301 | 0.67 | 0.929371 |
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Target: 5'- -aGAcGUGGggGCgcagGGCCucGGAGa -3' miRNA: 3'- gaCUaCACCuuCGacaaCCGGu-CCUC- -5' |
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| 29597 | 5' | -53.6 | NC_006151.1 | + | 45411 | 0.67 | 0.912105 |
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Target: 5'- gUGGUGccGGuguGUUGUgGGCCGGGGGu -3' miRNA: 3'- gACUACa-CCuu-CGACAaCCGGUCCUC- -5' |
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| 29597 | 5' | -53.6 | NC_006151.1 | + | 10972 | 0.67 | 0.903916 |
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Target: 5'- -cGGUGUGGugggugucacggugGAGCg---GGCCGGGGGc -3' miRNA: 3'- gaCUACACC--------------UUCGacaaCCGGUCCUC- -5' |
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| 29597 | 5' | -53.6 | NC_006151.1 | + | 29618 | 0.68 | 0.885573 |
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Target: 5'- gCUGGUGUGGGAGgaGUgggGGaauuGGGGg -3' miRNA: 3'- -GACUACACCUUCgaCAa--CCggu-CCUC- -5' |
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| 29597 | 5' | -53.6 | NC_006151.1 | + | 106228 | 0.68 | 0.885573 |
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Target: 5'- cCUGAUgGUGGccGCgg-UGGCCGGcGAGc -3' miRNA: 3'- -GACUA-CACCuuCGacaACCGGUC-CUC- -5' |
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| 29597 | 5' | -53.6 | NC_006151.1 | + | 29427 | 0.69 | 0.863158 |
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Target: 5'- -cGGUGUGGccGCggggGgcGGCgGGGAGa -3' miRNA: 3'- gaCUACACCuuCGa---CaaCCGgUCCUC- -5' |
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| 29597 | 5' | -53.6 | NC_006151.1 | + | 3826 | 0.69 | 0.855235 |
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Target: 5'- uUGGUGcUGGcgGGGCUGgagGGCCcGGAGc -3' miRNA: 3'- gACUAC-ACC--UUCGACaa-CCGGuCCUC- -5' |
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| 29597 | 5' | -53.6 | NC_006151.1 | + | 33001 | 0.71 | 0.739729 |
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Target: 5'- uUGGcUGUGGGuugucGGCUGUUGGCCGuuggccgaggacgacGGGGa -3' miRNA: 3'- gACU-ACACCU-----UCGACAACCGGU---------------CCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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