Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29603 | 3' | -59.4 | NC_006151.1 | + | 18302 | 0.69 | 0.558914 |
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Target: 5'- gUCgaagCCgGAGCGGgGCGCCUCCUcggGGCg -3' miRNA: 3'- aGGa---GG-CUCGCCaUGUGGAGGA---CCGa -5' |
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| 29603 | 3' | -59.4 | NC_006151.1 | + | 58963 | 0.7 | 0.539269 |
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Target: 5'- cCCUCgGugcGCGGccACGCCUCCgcgGGCa -3' miRNA: 3'- aGGAGgCu--CGCCa-UGUGGAGGa--CCGa -5' |
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| 29603 | 3' | -59.4 | NC_006151.1 | + | 113516 | 0.7 | 0.510265 |
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Target: 5'- -gCUUCGuGGCGGacgcGCGCUUCCUGGCg -3' miRNA: 3'- agGAGGC-UCGCCa---UGUGGAGGACCGa -5' |
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| 29603 | 3' | -59.4 | NC_006151.1 | + | 40243 | 0.7 | 0.491294 |
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Target: 5'- cUCCgCUGAGCGGgGCGCCcCCUcGGCc -3' miRNA: 3'- -AGGaGGCUCGCCaUGUGGaGGA-CCGa -5' |
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| 29603 | 3' | -59.4 | NC_006151.1 | + | 81188 | 0.71 | 0.481932 |
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Target: 5'- gCCgCCGAGCGuGUACGCCgggccgcagUCCgGGCg -3' miRNA: 3'- aGGaGGCUCGC-CAUGUGG---------AGGaCCGa -5' |
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| 29603 | 3' | -59.4 | NC_006151.1 | + | 134977 | 0.71 | 0.436499 |
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Target: 5'- gCCUCCGGGCcuucucGGgcggGCGCgUCUUGGCg -3' miRNA: 3'- aGGAGGCUCG------CCa---UGUGgAGGACCGa -5' |
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| 29603 | 3' | -59.4 | NC_006151.1 | + | 98614 | 0.73 | 0.380502 |
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Target: 5'- gCCUCgugagCGAGUGGUGCGCCgccuucgacgaggcCCUGGCg -3' miRNA: 3'- aGGAG-----GCUCGCCAUGUGGa-------------GGACCGa -5' |
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| 29603 | 3' | -59.4 | NC_006151.1 | + | 117783 | 0.73 | 0.377266 |
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Target: 5'- -gCUCCGGGCGGgGCGCCacgCCcGGCg -3' miRNA: 3'- agGAGGCUCGCCaUGUGGa--GGaCCGa -5' |
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| 29603 | 3' | -59.4 | NC_006151.1 | + | 100594 | 0.73 | 0.377266 |
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Target: 5'- gUCCUgCGGcccGUGGaGCGCUUCCUGGCg -3' miRNA: 3'- -AGGAgGCU---CGCCaUGUGGAGGACCGa -5' |
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| 29603 | 3' | -59.4 | NC_006151.1 | + | 68379 | 0.73 | 0.361364 |
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Target: 5'- gUCCUCCGucagggccGCGGc-CACCUCCaUGGCg -3' miRNA: 3'- -AGGAGGCu-------CGCCauGUGGAGG-ACCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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