Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2962 | 3' | -53.6 | NC_001493.1 | + | 24615 | 0.71 | 0.794708 |
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Target: 5'- cCCCgguUUCGucCACCGAGUCCAUCUu -3' miRNA: 3'- cGGGa--AGGUuaGUGGCUCAGGUAGGc -5' |
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| 2962 | 3' | -53.6 | NC_001493.1 | + | 131731 | 0.71 | 0.776034 |
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Target: 5'- -aCCUUCCcgcacUCACCGAgaccuuccuGUCCAUCCu -3' miRNA: 3'- cgGGAAGGuu---AGUGGCU---------CAGGUAGGc -5' |
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| 2962 | 3' | -53.6 | NC_001493.1 | + | 93673 | 0.72 | 0.747049 |
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Target: 5'- cGCCCg-CCGAUUuacccCCGGGUCCGUUCa -3' miRNA: 3'- -CGGGaaGGUUAGu----GGCUCAGGUAGGc -5' |
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| 2962 | 3' | -53.6 | NC_001493.1 | + | 7661 | 0.72 | 0.727188 |
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Target: 5'- cGCCCUcCCGAUcCACCGGGcCCGaaaCCGc -3' miRNA: 3'- -CGGGAaGGUUA-GUGGCUCaGGUa--GGC- -5' |
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| 2962 | 3' | -53.6 | NC_001493.1 | + | 123216 | 0.72 | 0.727188 |
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Target: 5'- cGCCCUcCCGAUcCACCGGGcCCGaaaCCGc -3' miRNA: 3'- -CGGGAaGGUUA-GUGGCUCaGGUa--GGC- -5' |
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| 2962 | 3' | -53.6 | NC_001493.1 | + | 53012 | 0.73 | 0.645121 |
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Target: 5'- cCCUUUCC--UgGCCGAGUCCAUCaCGu -3' miRNA: 3'- cGGGAAGGuuAgUGGCUCAGGUAG-GC- -5' |
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| 2962 | 3' | -53.6 | NC_001493.1 | + | 26901 | 0.74 | 0.603539 |
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Target: 5'- aGUCCgagUCCGAgu-CCGAGUCCgaGUCCGa -3' miRNA: 3'- -CGGGa--AGGUUaguGGCUCAGG--UAGGC- -5' |
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| 2962 | 3' | -53.6 | NC_001493.1 | + | 85696 | 1.11 | 0.00323 |
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Target: 5'- gGCCCUUCCAAUCACCGAGUCCAUCCGa -3' miRNA: 3'- -CGGGAAGGUUAGUGGCUCAGGUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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