Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29625 | 3' | -51.5 | NC_006151.1 | + | 32280 | 0.66 | 0.99306 |
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Target: 5'- --uUUCCUUCcUCUcCUUCUUCCUCc -3' miRNA: 3'- ccuGAGGAAGcAGAaGAAGAGGGAGc -5' |
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| 29625 | 3' | -51.5 | NC_006151.1 | + | 25485 | 0.66 | 0.992961 |
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Target: 5'- aGACUcgcgccgCCUcCGUCcUCUcUCUCCCUCc -3' miRNA: 3'- cCUGA-------GGAaGCAGaAGA-AGAGGGAGc -5' |
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| 29625 | 3' | -51.5 | NC_006151.1 | + | 134476 | 0.66 | 0.991574 |
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Target: 5'- cGGACggccgccacugggCCUaCGUCUUCgggcgcUCgUCCCUCa -3' miRNA: 3'- -CCUGa------------GGAaGCAGAAGa-----AG-AGGGAGc -5' |
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| 29625 | 3' | -51.5 | NC_006151.1 | + | 26420 | 0.66 | 0.989579 |
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Target: 5'- --uCUCCgucuUCGUCUUCgUCUUCUUCa -3' miRNA: 3'- ccuGAGGa---AGCAGAAGaAGAGGGAGc -5' |
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| 29625 | 3' | -51.5 | NC_006151.1 | + | 46766 | 0.66 | 0.986588 |
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Target: 5'- cGGcucGCUCCUcccccUCGUCUUCaccCUCcuCCUCGa -3' miRNA: 3'- -CC---UGAGGA-----AGCAGAAGaa-GAG--GGAGC- -5' |
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| 29625 | 3' | -51.5 | NC_006151.1 | + | 101608 | 0.66 | 0.986588 |
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Target: 5'- uGGGCcggggCCUUCGUgUUCUUCcugCCC-Ca -3' miRNA: 3'- -CCUGa----GGAAGCAgAAGAAGa--GGGaGc -5' |
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| 29625 | 3' | -51.5 | NC_006151.1 | + | 26733 | 0.68 | 0.967751 |
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Target: 5'- --cCUCC-UCGUCUUCUUCcucuuccCCCUCc -3' miRNA: 3'- ccuGAGGaAGCAGAAGAAGa------GGGAGc -5' |
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| 29625 | 3' | -51.5 | NC_006151.1 | + | 40318 | 0.72 | 0.840709 |
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Target: 5'- aGGcccuuCUCCUcCGUCUUC-UCUCcCCUCGc -3' miRNA: 3'- -CCu----GAGGAaGCAGAAGaAGAG-GGAGC- -5' |
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| 29625 | 3' | -51.5 | NC_006151.1 | + | 5150 | 1.13 | 0.004671 |
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Target: 5'- cGGACUCCUUCGUCUUCUUCUCCCUCGg -3' miRNA: 3'- -CCUGAGGAAGCAGAAGAAGAGGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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