Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29626 | 5' | -58.7 | NC_006151.1 | + | 141273 | 0.69 | 0.584938 |
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Target: 5'- -cGGGGCCUCgGCGGcUGCGG---UCCGa -3' miRNA: 3'- uaCCCCGGAG-UGCC-ACGUUcgaAGGC- -5' |
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| 29626 | 5' | -58.7 | NC_006151.1 | + | 65717 | 0.69 | 0.564725 |
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Target: 5'- -gGGGGCCUCgcGCGGcUGCGcGCggCCc -3' miRNA: 3'- uaCCCCGGAG--UGCC-ACGUuCGaaGGc -5' |
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| 29626 | 5' | -58.7 | NC_006151.1 | + | 131923 | 0.69 | 0.554685 |
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Target: 5'- --aGGGCCUcCGCGGUGCugccguAGCUggCCa -3' miRNA: 3'- uacCCCGGA-GUGCCACGu-----UCGAa-GGc -5' |
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| 29626 | 5' | -58.7 | NC_006151.1 | + | 19504 | 0.72 | 0.430864 |
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Target: 5'- -cGGGGCCUCGgGGacGCAGGCgcggCCc -3' miRNA: 3'- uaCCCCGGAGUgCCa-CGUUCGaa--GGc -5' |
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| 29626 | 5' | -58.7 | NC_006151.1 | + | 19294 | 0.72 | 0.413251 |
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Target: 5'- -cGGGGCCa-GCGGcGCGGGCggCCGg -3' miRNA: 3'- uaCCCCGGagUGCCaCGUUCGaaGGC- -5' |
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| 29626 | 5' | -58.7 | NC_006151.1 | + | 52382 | 0.73 | 0.355246 |
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Target: 5'- -cGGGGCC-CGCGGgggcgGCGGGCgccgCCGc -3' miRNA: 3'- uaCCCCGGaGUGCCa----CGUUCGaa--GGC- -5' |
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| 29626 | 5' | -58.7 | NC_006151.1 | + | 723 | 0.73 | 0.355246 |
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Target: 5'- -gGGGGUC-CGCGG-GCGggGGCUUCCGc -3' miRNA: 3'- uaCCCCGGaGUGCCaCGU--UCGAAGGC- -5' |
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| 29626 | 5' | -58.7 | NC_006151.1 | + | 4039 | 0.73 | 0.33976 |
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Target: 5'- -aGGcGGCCUCGCGGgcGCGGGCcccgUCCa -3' miRNA: 3'- uaCC-CCGGAGUGCCa-CGUUCGa---AGGc -5' |
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| 29626 | 5' | -58.7 | NC_006151.1 | + | 7290 | 1.06 | 0.002051 |
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Target: 5'- aAUGGGGCCUCACGGUGCAAGCUUCCGu -3' miRNA: 3'- -UACCCCGGAGUGCCACGUUCGAAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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