miRNA: 3'- uACGGG-G-GCUC--------C-GGGGGCUCCCGCuc -5'
miRNA: 3'- uaCGGGGGCuCCGGGGGcuCCCGCUC- -5'
miRNA: 3'- uACGGGgGCUC-----CGGGGGCUCCCGCUc -5'
miRNA: 3'- uaCGGGg-GCUCCGGgGGCU----CCCGCUC- -5'
miRNA: 3'- uacGGGGGCuCCGGGGG--------------CUCCCGcuc -5'
Results 121 - 125 of 125 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29635 | 3' | -67.6 | NC_006151.1 | + | 134411 | 0.67 | 0.316722 |
|
Target: 5'- cUGCCCgCgCGAGcuggugcuGcCCCCCGGGGGCGc- -3' miRNA: 3'- uACGGG-G-GCUC--------C-GGGGGCUCCCGCuc -5' |
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| 29635 | 3' | -67.6 | NC_006151.1 | + | 134976 | 0.7 | 0.207127 |
|
Target: 5'- -aGCCUCCG-GGCCUUCucGGGCGGGc -3' miRNA: 3'- uaCGGGGGCuCCGGGGGcuCCCGCUC- -5' |
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| 29635 | 3' | -67.6 | NC_006151.1 | + | 135717 | 0.67 | 0.323561 |
|
Target: 5'- cUGCUCaCGGGcaggaGCCCUCGGGGGCGGu -3' miRNA: 3'- uACGGGgGCUC-----CGGGGGCUCCCGCUc -5' |
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| 29635 | 3' | -67.6 | NC_006151.1 | + | 141606 | 0.69 | 0.254321 |
|
Target: 5'- -cGCCCggCGAGGCUgCCGGgaucGGGCGGGu -3' miRNA: 3'- uaCGGGg-GCUCCGGgGGCU----CCCGCUC- -5' |
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| 29635 | 3' | -67.6 | NC_006151.1 | + | 142722 | 0.66 | 0.372186 |
|
Target: 5'- --uCCCCCG-GGCCCCCacaacucucuggccGGGGGCc-- -3' miRNA: 3'- uacGGGGGCuCCGGGGG--------------CUCCCGcuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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