miRNA: 3'- uaCGGGGGCUCCG------GGGGCUC-C-CGCUc -5'
miRNA: 3'- uaCGGGGGCUCCGGgGgCUC---CCGCUC- -5'
miRNA: 3'- uaCGgGGGCUCCGGGGGCuCCCGcUC- -5'
miRNA: 3'- uaCGGGGGCUCCGgG--GGCuCCCGCUC- -5'
miRNA: 3'- uaCG-GGGGCUCCgGGgGCU-------CCCGCUC- -5'
Results 121 - 125 of 125 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29635 | 3' | -67.6 | NC_006151.1 | + | 3674 | 0.69 | 0.237677 |
|
Target: 5'- -cGUCCCCGGGGCggcgggCCCCGGGcGcGCGGc -3' miRNA: 3'- uaCGGGGGCUCCG------GGGGCUC-C-CGCUc -5' |
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| 29635 | 3' | -67.6 | NC_006151.1 | + | 2394 | 0.74 | 0.100735 |
|
Target: 5'- -gGCCCUCGGGGCCgCgGAGcucGGCGAGg -3' miRNA: 3'- uaCGGGGGCUCCGGgGgCUC---CCGCUC- -5' |
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| 29635 | 3' | -67.6 | NC_006151.1 | + | 2188 | 0.79 | 0.048635 |
|
Target: 5'- -gGCgCCCGAGGCCCCCGcGGGCcGGu -3' miRNA: 3'- uaCGgGGGCUCCGGGGGCuCCCGcUC- -5' |
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| 29635 | 3' | -67.6 | NC_006151.1 | + | 650 | 0.67 | 0.337565 |
|
Target: 5'- -gGCUUCCGGGGCgCggCCG-GGGCGGGc -3' miRNA: 3'- uaCGGGGGCUCCGgG--GGCuCCCGCUC- -5' |
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| 29635 | 3' | -67.6 | NC_006151.1 | + | 10 | 0.71 | 0.188253 |
|
Target: 5'- -cGCuCCCCGGGGgCCgCGAaaaaaggGGGCGGGg -3' miRNA: 3'- uaCG-GGGGCUCCgGGgGCU-------CCCGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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