Results 121 - 125 of 125 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29635 | 3' | -67.6 | NC_006151.1 | + | 19287 | 0.67 | 0.316722 |
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Target: 5'- -gGCCCaCCGGGGCCagCGGcgcGGGCGGc -3' miRNA: 3'- uaCGGG-GGCUCCGGggGCU---CCCGCUc -5' |
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| 29635 | 3' | -67.6 | NC_006151.1 | + | 40013 | 0.67 | 0.312672 |
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Target: 5'- uUGCCCCCuucGAGaccagcguccgcgccGCCgCCGGGGGCGc- -3' miRNA: 3'- uACGGGGG---CUC---------------CGGgGGCUCCCGCuc -5' |
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| 29635 | 3' | -67.6 | NC_006151.1 | + | 14027 | 0.67 | 0.309994 |
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Target: 5'- -gGCCCgUCGGcccGGCCCCCucgacGGGGCGAu -3' miRNA: 3'- uaCGGG-GGCU---CCGGGGGc----UCCCGCUc -5' |
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| 29635 | 3' | -67.6 | NC_006151.1 | + | 114921 | 0.67 | 0.303374 |
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Target: 5'- -cGgCCCCGGcgucgcgcucGGCCUCCG-GGGUGGGc -3' miRNA: 3'- uaCgGGGGCU----------CCGGGGGCuCCCGCUC- -5' |
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| 29635 | 3' | -67.6 | NC_006151.1 | + | 22626 | 0.68 | 0.284171 |
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Target: 5'- gAUGCCCacgaCGAccaGGCCggCGAGGGCGAc -3' miRNA: 3'- -UACGGGg---GCU---CCGGggGCUCCCGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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