Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29635 | 5' | -56.1 | NC_006151.1 | + | 107599 | 0.66 | 0.891702 |
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Target: 5'- aAGCUGGCCccgCCGGCGccgccgcccgccaaGCCcguggaGACGACc -3' miRNA: 3'- -UCGACUGGa--GGUCGC--------------UGGag----CUGUUG- -5' |
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| 29635 | 5' | -56.1 | NC_006151.1 | + | 116777 | 0.66 | 0.893719 |
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Target: 5'- cAGCgUGugCUCCAGCcGCUgggUGGCGAg -3' miRNA: 3'- -UCG-ACugGAGGUCGcUGGa--GCUGUUg -5' |
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| 29635 | 5' | -56.1 | NC_006151.1 | + | 77608 | 0.66 | 0.893719 |
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Target: 5'- gAGCUgGACCUCU-GCGGCCgcgCGG-AGCu -3' miRNA: 3'- -UCGA-CUGGAGGuCGCUGGa--GCUgUUG- -5' |
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| 29635 | 5' | -56.1 | NC_006151.1 | + | 35740 | 0.66 | 0.893719 |
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Target: 5'- ---cGGCuCUCCGGCGGcuaucagcCCUCGACGGa -3' miRNA: 3'- ucgaCUG-GAGGUCGCU--------GGAGCUGUUg -5' |
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| 29635 | 5' | -56.1 | NC_006151.1 | + | 39591 | 0.66 | 0.893719 |
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Target: 5'- cGCUGcGCCUCU-GCcGCCagGACAACg -3' miRNA: 3'- uCGAC-UGGAGGuCGcUGGagCUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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