Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29637 | 5' | -53.8 | NC_006151.1 | + | 84228 | 0.69 | 0.870119 |
Target: 5'- -gUGCCC-CUGcugGUgUUCGUGGCCCa -3' miRNA: 3'- gaGCGGGaGACug-UAgAAGCACUGGG- -5' |
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29637 | 5' | -53.8 | NC_006151.1 | + | 77546 | 0.69 | 0.859474 |
Target: 5'- -cCGCCC-CgcaggggagcgacGACGUCUUCGUGgacGCCCu -3' miRNA: 3'- gaGCGGGaGa------------CUGUAGAAGCAC---UGGG- -5' |
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29637 | 5' | -53.8 | NC_006151.1 | + | 139292 | 0.7 | 0.846805 |
Target: 5'- -cCGCCCUC-GGCGUCUgcggcgCGUGcuGCUCg -3' miRNA: 3'- gaGCGGGAGaCUGUAGAa-----GCAC--UGGG- -5' |
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29637 | 5' | -53.8 | NC_006151.1 | + | 70006 | 0.7 | 0.846805 |
Target: 5'- -aCGCUCUCgacGGCgcccGUCUgcaCGUGGCCCg -3' miRNA: 3'- gaGCGGGAGa--CUG----UAGAa--GCACUGGG- -5' |
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29637 | 5' | -53.8 | NC_006151.1 | + | 135998 | 0.71 | 0.795018 |
Target: 5'- uUCGCCCUCcacGACGgccUCaUCGcGGCCCu -3' miRNA: 3'- gAGCGGGAGa--CUGU---AGaAGCaCUGGG- -5' |
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29637 | 5' | -53.8 | NC_006151.1 | + | 23242 | 0.71 | 0.765073 |
Target: 5'- cCUCGCCCUCgggggccucgggGGCAUCgcCGUGGacgaCCu -3' miRNA: 3'- -GAGCGGGAGa-----------CUGUAGaaGCACUg---GG- -5' |
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29637 | 5' | -53.8 | NC_006151.1 | + | 86861 | 0.72 | 0.727935 |
Target: 5'- -gCGCCCggcgaCU-ACAUCUUCGUG-CCCg -3' miRNA: 3'- gaGCGGGa----GAcUGUAGAAGCACuGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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