Results 121 - 127 of 127 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29638 | 3' | -62.1 | NC_006151.1 | + | 17330 | 0.67 | 0.54146 |
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Target: 5'- cGCCGCGUCaGCUCgugcgUCUCGGUGGUg-- -3' miRNA: 3'- -CGGUGUAGgCGAG-----GGAGCCGCCGgag -5' |
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| 29638 | 3' | -62.1 | NC_006151.1 | + | 107504 | 0.67 | 0.54146 |
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Target: 5'- cGCCGCcgGUCgacccgcccaCGCUCCCgccguuggcccCGGCGGCCcCg -3' miRNA: 3'- -CGGUG--UAG----------GCGAGGGa----------GCCGCCGGaG- -5' |
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| 29638 | 3' | -62.1 | NC_006151.1 | + | 67995 | 0.67 | 0.54146 |
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Target: 5'- cGCCgGCG-CCGCggCCacgggCUCGGCGGCgUCc -3' miRNA: 3'- -CGG-UGUaGGCGa-GG-----GAGCCGCCGgAG- -5' |
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| 29638 | 3' | -62.1 | NC_006151.1 | + | 103183 | 0.67 | 0.54146 |
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Target: 5'- cGCCAUggaCGCcgCCCU-GGCGGCCg- -3' miRNA: 3'- -CGGUGuagGCGa-GGGAgCCGCCGGag -5' |
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| 29638 | 3' | -62.1 | NC_006151.1 | + | 101843 | 0.67 | 0.531889 |
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Target: 5'- cGCCGCccCCGC-CCCggcaccCGGCGGCg-- -3' miRNA: 3'- -CGGUGuaGGCGaGGGa-----GCCGCCGgag -5' |
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| 29638 | 3' | -62.1 | NC_006151.1 | + | 62008 | 0.67 | 0.531889 |
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Target: 5'- gGUgACGUgCGCgccgCCCU-GGCGGCCa- -3' miRNA: 3'- -CGgUGUAgGCGa---GGGAgCCGCCGGag -5' |
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| 29638 | 3' | -62.1 | NC_006151.1 | + | 133115 | 0.68 | 0.483221 |
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Target: 5'- cGCCGCGUCCGuCgucgggggguucaugUCCCagagggacUCGGCggcggcgcgggcgugGGCCUCg -3' miRNA: 3'- -CGGUGUAGGC-G---------------AGGG--------AGCCG---------------CCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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