miRNA: 3'- -UGCUGUgCUCGGCCuuCUGCCaCGUu -5'
miRNA: 3'- -UGCUgUGCUCGGCCU---UCUGCCaCGUU- -5'
miRNA: 3'- -UGCUGUgCUC-GGCCuuCUGCCa--CGUU- -5'
miRNA: 3'- -UGCuGUGCUCG---GCCUUCUGCCACGUU- -5'
miRNA: 3'- -UGCUGUGCUCGGCCUUCUGCCACGUU- -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29648 | 5' | -56.8 | NC_006151.1 | + | 20122 | 0.75 | 0.388965 |
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Target: 5'- gGCGACG-GGGCUGGggGGCGG-GCGc -3' miRNA: 3'- -UGCUGUgCUCGGCCuuCUGCCaCGUu -5' |
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| 29648 | 5' | -56.8 | NC_006151.1 | + | 135123 | 0.75 | 0.364248 |
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Target: 5'- cGCGGgGCGGGCUGGGcggGGACGG-GCAGa -3' miRNA: 3'- -UGCUgUGCUCGGCCU---UCUGCCaCGUU- -5' |
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| 29648 | 5' | -56.8 | NC_006151.1 | + | 5251 | 0.77 | 0.296813 |
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Target: 5'- gGCGGCGgGGGcCCGGggGGCGGaggGCGAg -3' miRNA: 3'- -UGCUGUgCUC-GGCCuuCUGCCa--CGUU- -5' |
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| 29648 | 5' | -56.8 | NC_006151.1 | + | 99194 | 0.8 | 0.173284 |
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Target: 5'- gACGcCACGGGCgugUGGGAGGCGGUGCAGg -3' miRNA: 3'- -UGCuGUGCUCG---GCCUUCUGCCACGUU- -5' |
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| 29648 | 5' | -56.8 | NC_006151.1 | + | 34293 | 1.05 | 0.003678 |
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Target: 5'- gACGACACGAGCCGGAAGACGGUGCAAc -3' miRNA: 3'- -UGCUGUGCUCGGCCUUCUGCCACGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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