Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29671 | 3' | -56.9 | NC_006261.1 | + | 1212 | 0.66 | 0.080406 |
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Target: 5'- -uUCCCGAacuuuuucugAGCCC--AGCCGagaaagacggaGCCGGCg -3' miRNA: 3'- ucGGGGCU----------UCGGGuuUCGGU-----------UGGUCG- -5' |
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| 29671 | 3' | -56.9 | NC_006261.1 | + | 1385 | 0.66 | 0.072392 |
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Target: 5'- aGGUCgUGGAGUccgCCAAGGCCAuucUCGGCg -3' miRNA: 3'- -UCGGgGCUUCG---GGUUUCGGUu--GGUCG- -5' |
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| 29671 | 3' | -56.9 | NC_006261.1 | + | 3512 | 0.67 | 0.065148 |
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Target: 5'- cAGCCaaGGugGGCCUAAuacaauGGCCAAUCAGg -3' miRNA: 3'- -UCGGggCU--UCGGGUU------UCGGUUGGUCg -5' |
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| 29671 | 3' | -56.9 | NC_006261.1 | + | 3398 | 0.67 | 0.058606 |
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Target: 5'- cGCCCac-AG-CCAGAGCUugGACCGGCu -3' miRNA: 3'- uCGGGgcuUCgGGUUUCGG--UUGGUCG- -5' |
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| 29671 | 3' | -56.9 | NC_006261.1 | + | 458 | 0.68 | 0.052702 |
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Target: 5'- gGGCCuCCGggGCCUuacucacgcGgcGCCAuuggACCAGg -3' miRNA: 3'- -UCGG-GGCuuCGGG---------UuuCGGU----UGGUCg -5' |
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| 29671 | 3' | -56.9 | NC_006261.1 | + | 3530 | 0.7 | 0.033157 |
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Target: 5'- aGGCCCaccuuggcUGAAGCCCAGAGUCugcguauuuGCCGuGCc -3' miRNA: 3'- -UCGGG--------GCUUCGGGUUUCGGu--------UGGU-CG- -5' |
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| 29671 | 3' | -56.9 | NC_006261.1 | + | 941 | 0.72 | 0.020781 |
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Target: 5'- uGCCCCG--GUCCAccAGCCAcccgACCAGCu -3' miRNA: 3'- uCGGGGCuuCGGGUu-UCGGU----UGGUCG- -5' |
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| 29671 | 3' | -56.9 | NC_006261.1 | + | 2330 | 0.78 | 0.007016 |
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Target: 5'- cGGCCCUcGAGCacgaCAAGGCCuacGACCAGCa -3' miRNA: 3'- -UCGGGGcUUCGg---GUUUCGG---UUGGUCG- -5' |
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| 29671 | 3' | -56.9 | NC_006261.1 | + | 2201 | 1.12 | 5e-06 |
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Target: 5'- gAGCCCCGAAGCCCAAAGCCAACCAGCa -3' miRNA: 3'- -UCGGGGCUUCGGGUUUCGGUUGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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