Results 21 - 30 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2968 | 3' | -59.9 | NC_001493.1 | + | 5154 | 0.69 | 0.564061 |
Target: 5'- -cCGGUCACCGgggccgagcgugcAGCCGGuGgacUUCCUCGu -3' miRNA: 3'- guGCCGGUGGC-------------UCGGCCuC---AAGGAGC- -5' |
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2968 | 3' | -59.9 | NC_001493.1 | + | 120709 | 0.69 | 0.564061 |
Target: 5'- -cCGGUCACCGgggccgagcgugcAGCCGGuGgacUUCCUCGu -3' miRNA: 3'- guGCCGGUGGC-------------UCGGCCuC---AAGGAGC- -5' |
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2968 | 3' | -59.9 | NC_001493.1 | + | 96008 | 0.7 | 0.488323 |
Target: 5'- gGCGGUCGCgGGcGCCGuGAGacucUUCCUCGu -3' miRNA: 3'- gUGCCGGUGgCU-CGGC-CUC----AAGGAGC- -5' |
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2968 | 3' | -59.9 | NC_001493.1 | + | 49178 | 0.7 | 0.469888 |
Target: 5'- gACGGCCGCCaugcGCCGcuccAGUUCCUCc -3' miRNA: 3'- gUGCCGGUGGcu--CGGCc---UCAAGGAGc -5' |
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2968 | 3' | -59.9 | NC_001493.1 | + | 10851 | 0.7 | 0.468976 |
Target: 5'- cCAUGGCCGCCgucauauuagagcGAGCCGccGAGUUUgUCGc -3' miRNA: 3'- -GUGCCGGUGG-------------CUCGGC--CUCAAGgAGC- -5' |
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2968 | 3' | -59.9 | NC_001493.1 | + | 126405 | 0.7 | 0.468976 |
Target: 5'- cCAUGGCCGCCgucauauuagagcGAGCCGccGAGUUUgUCGc -3' miRNA: 3'- -GUGCCGGUGG-------------CUCGGC--CUCAAGgAGC- -5' |
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2968 | 3' | -59.9 | NC_001493.1 | + | 90833 | 0.72 | 0.375553 |
Target: 5'- aACGGCCucucgacuCgGAGCCGGcGggCCUCGa -3' miRNA: 3'- gUGCCGGu-------GgCUCGGCCuCaaGGAGC- -5' |
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2968 | 3' | -59.9 | NC_001493.1 | + | 8021 | 0.73 | 0.337111 |
Target: 5'- uCGgGGCCACCGGGCgGGAGgggaUCGa -3' miRNA: 3'- -GUgCCGGUGGCUCGgCCUCaaggAGC- -5' |
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2968 | 3' | -59.9 | NC_001493.1 | + | 123575 | 0.73 | 0.337111 |
Target: 5'- uCGgGGCCACCGGGCgGGAGgggaUCGa -3' miRNA: 3'- -GUgCCGGUGGCUCGgCCUCaaggAGC- -5' |
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2968 | 3' | -59.9 | NC_001493.1 | + | 88996 | 1.08 | 0.00134 |
Target: 5'- cCACGGCCACCGAGCCGGAGUUCCUCGa -3' miRNA: 3'- -GUGCCGGUGGCUCGGCCUCAAGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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