Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2968 | 5' | -56.8 | NC_001493.1 | + | 64081 | 0.66 | 0.871716 |
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Target: 5'- gUCGGGGGCGCGUuCCGA---GG-GAu -3' miRNA: 3'- aAGCUCCCGUGCAuGGCUgagCCaCU- -5' |
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| 2968 | 5' | -56.8 | NC_001493.1 | + | 27609 | 0.66 | 0.864259 |
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Target: 5'- cUCGAGGGgACccgcGCgGGCUCGG-GAu -3' miRNA: 3'- aAGCUCCCgUGca--UGgCUGAGCCaCU- -5' |
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| 2968 | 5' | -56.8 | NC_001493.1 | + | 88834 | 0.66 | 0.832392 |
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Target: 5'- gUCGAGGa-ACu--CCGGCUCGGUGGc -3' miRNA: 3'- aAGCUCCcgUGcauGGCUGAGCCACU- -5' |
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| 2968 | 5' | -56.8 | NC_001493.1 | + | 3743 | 0.66 | 0.832392 |
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Target: 5'- -cCGAGG-CGCGUACCG-CUCugaucgagugGGUGGa -3' miRNA: 3'- aaGCUCCcGUGCAUGGCuGAG----------CCACU- -5' |
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| 2968 | 5' | -56.8 | NC_001493.1 | + | 119297 | 0.66 | 0.832392 |
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Target: 5'- -cCGAGG-CGCGUACCG-CUCugaucgagugGGUGGa -3' miRNA: 3'- aaGCUCCcGUGCAUGGCuGAG----------CCACU- -5' |
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| 2968 | 5' | -56.8 | NC_001493.1 | + | 34871 | 0.67 | 0.815331 |
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Target: 5'- -aCGAGGGCGuacaaccaccccCGU-UCGACcCGGUGAa -3' miRNA: 3'- aaGCUCCCGU------------GCAuGGCUGaGCCACU- -5' |
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| 2968 | 5' | -56.8 | NC_001493.1 | + | 106156 | 0.67 | 0.806546 |
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Target: 5'- -gUGGGGGCAgGUGCCcAUggaGGUGAc -3' miRNA: 3'- aaGCUCCCGUgCAUGGcUGag-CCACU- -5' |
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| 2968 | 5' | -56.8 | NC_001493.1 | + | 87778 | 0.68 | 0.731285 |
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Target: 5'- cUCGAcGGGCAC-UACCGGCgCGGa-- -3' miRNA: 3'- aAGCU-CCCGUGcAUGGCUGaGCCacu -5' |
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| 2968 | 5' | -56.8 | NC_001493.1 | + | 18851 | 0.71 | 0.568692 |
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Target: 5'- -gUGAGGGguCGUGcCCGAC-CGGUGu -3' miRNA: 3'- aaGCUCCCguGCAU-GGCUGaGCCACu -5' |
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| 2968 | 5' | -56.8 | NC_001493.1 | + | 88962 | 1.07 | 0.003081 |
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Target: 5'- gUUCGAGGGCACGUACCGACUCGGUGAg -3' miRNA: 3'- -AAGCUCCCGUGCAUGGCUGAGCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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