miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
29684 5' -46.9 NC_006265.1 + 4222 0.66 0.505085
Target:  5'- aUGuuGCUuCGGAGuGggUUCUGGGGu- -3'
miRNA:   3'- -ACggCGGuGUUUU-CuuAAGAUCCUuc -5'
29684 5' -46.9 NC_006265.1 + 2949 0.69 0.297741
Target:  5'- gGCUGCCAUAAcccGGAUUCUAGaauuaGAGGa -3'
miRNA:   3'- aCGGCGGUGUUuu-CUUAAGAUC-----CUUC- -5'
29684 5' -46.9 NC_006265.1 + 3270 1.11 0.000205
Target:  5'- gUGCCGCCACAAAAGAAUUCUAGGAAGa -3'
miRNA:   3'- -ACGGCGGUGUUUUCUUAAGAUCCUUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.