Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29705 | 5' | -47.9 | NC_006271.1 | + | 2961 | 0.66 | 0.471746 |
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Target: 5'- -cGCUAUagucCUGCUUUGG-GGCAGCAc -3' miRNA: 3'- cuUGAUAau--GACGGAACCgUCGUUGU- -5' |
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| 29705 | 5' | -47.9 | NC_006271.1 | + | 1289 | 0.69 | 0.313527 |
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Target: 5'- --cCUAUacaUGCUGCCUUuucaGGCAGCAc-- -3' miRNA: 3'- cuuGAUA---AUGACGGAA----CCGUCGUugu -5' |
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| 29705 | 5' | -47.9 | NC_006271.1 | + | 1249 | 0.69 | 0.303892 |
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Target: 5'- --uCUGgggUGCUGCCUgaaaaGGCAGCAu-- -3' miRNA: 3'- cuuGAUa--AUGACGGAa----CCGUCGUugu -5' |
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| 29705 | 5' | -47.9 | NC_006271.1 | + | 224 | 0.72 | 0.185862 |
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Target: 5'- ------cUGCUGCCUaccaGGCAGCAACu -3' miRNA: 3'- cuugauaAUGACGGAa---CCGUCGUUGu -5' |
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| 29705 | 5' | -47.9 | NC_006271.1 | + | 252 | 0.74 | 0.141032 |
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Target: 5'- ----aGUUGCUGCCUgguaGGCAGCAGa- -3' miRNA: 3'- cuugaUAAUGACGGAa---CCGUCGUUgu -5' |
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| 29705 | 5' | -47.9 | NC_006271.1 | + | 592 | 0.84 | 0.021121 |
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Target: 5'- -cGCUGUUGCUGCCaaGGCAGUAAUAg -3' miRNA: 3'- cuUGAUAAUGACGGaaCCGUCGUUGU- -5' |
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| 29705 | 5' | -47.9 | NC_006271.1 | + | 627 | 1.1 | 0.000222 |
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Target: 5'- uGAACUAUUACUGCCUUGGCAGCAACAg -3' miRNA: 3'- -CUUGAUAAUGACGGAACCGUCGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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