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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29709 | 3' | -47 | NC_006271.1 | + | 2091 | 0.67 | 0.43716 |
Target: 5'- gGGAUGCUUGAGCAggcUGCAgauuuGCa-- -3' miRNA: 3'- -UCUACGAAUUCGUga-ACGUau---CGacu -5' |
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29709 | 3' | -47 | NC_006271.1 | + | 4126 | 0.7 | 0.295575 |
Target: 5'- --uUGCUUAAGCACcaagugGUucugAUAGCUGAa -3' miRNA: 3'- ucuACGAAUUCGUGaa----CG----UAUCGACU- -5' |
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29709 | 3' | -47 | NC_006271.1 | + | 2474 | 1.1 | 0.000253 |
Target: 5'- aAGAUGCUUAAGCACUUGCAUAGCUGAu -3' miRNA: 3'- -UCUACGAAUUCGUGAACGUAUCGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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