miRNA: 3'- cuuuuGAGAUGGaUAGUUaGAGAGGC- -5'
miRNA: 3'- cUUUUGAGAUGGaUAGUUAGaGAGGc -5'
miRNA: 3'- -CUUUUGAGAUGGAUAGUUAGAGAGGC- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29715 | 3' | -44.7 | NC_006272.1 | + | 89 | 0.69 | 0.309928 |
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Target: 5'- ----uCUCUACC-GUCAAaCUCUCUGa -3' miRNA: 3'- cuuuuGAGAUGGaUAGUUaGAGAGGC- -5' |
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| 29715 | 3' | -44.7 | NC_006272.1 | + | 1527 | 0.72 | 0.204216 |
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Target: 5'- uGAAACUCUAUCaGUUAAUC-CUCCu -3' miRNA: 3'- cUUUUGAGAUGGaUAGUUAGaGAGGc -5' |
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| 29715 | 3' | -44.7 | NC_006272.1 | + | 1593 | 1.1 | 0.000219 |
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Target: 5'- aGAAAACUCUACCUAUCAAUCUCUCCGc -3' miRNA: 3'- -CUUUUGAGAUGGAUAGUUAGAGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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