Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29726 | 3' | -54.7 | NC_006273.1 | + | 81114 | 0.72 | 0.797616 |
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Target: 5'- aAAGCCGCGUCAuCUCGgCGG-CGUacgAGGg -3' miRNA: 3'- -UUUGGCGCAGU-GAGU-GCCuGCA---UCCa -5' |
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| 29726 | 3' | -54.7 | NC_006273.1 | + | 118315 | 0.72 | 0.779647 |
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Target: 5'- cAGCUuggGCaGUCGCUCACGGAUGUAGa- -3' miRNA: 3'- uUUGG---CG-CAGUGAGUGCCUGCAUCca -5' |
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| 29726 | 3' | -54.7 | NC_006273.1 | + | 213134 | 0.73 | 0.742243 |
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Target: 5'- gGAGCCGCGUCGCUCGCcGGCGc---- -3' miRNA: 3'- -UUUGGCGCAGUGAGUGcCUGCaucca -5' |
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| 29726 | 3' | -54.7 | NC_006273.1 | + | 187515 | 0.74 | 0.653491 |
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Target: 5'- gAAGCCGCGUCGgUCACaGGACGgcGa- -3' miRNA: 3'- -UUUGGCGCAGUgAGUG-CCUGCauCca -5' |
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| 29726 | 3' | -54.7 | NC_006273.1 | + | 51918 | 0.75 | 0.633374 |
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Target: 5'- uGugCGCGU-GCUgACGGACGUGGGa -3' miRNA: 3'- uUugGCGCAgUGAgUGCCUGCAUCCa -5' |
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| 29726 | 3' | -54.7 | NC_006273.1 | + | 209994 | 1.05 | 0.011232 |
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Target: 5'- gAAACCGCGUCACUCACGGACGUAGGUu -3' miRNA: 3'- -UUUGGCGCAGUGAGUGCCUGCAUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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