Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29726 | 3' | -54.7 | NC_006273.1 | + | 194981 | 0.66 | 0.970755 |
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Target: 5'- -uGCCGCGggugugUCGCGGGCGUgugccGGGUg -3' miRNA: 3'- uuUGGCGCagug--AGUGCCUGCA-----UCCA- -5' |
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| 29726 | 3' | -54.7 | NC_006273.1 | + | 201161 | 0.68 | 0.941443 |
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Target: 5'- --gUCGUGUCGCgaCAUGGACGUgcAGGg -3' miRNA: 3'- uuuGGCGCAGUGa-GUGCCUGCA--UCCa -5' |
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| 29726 | 3' | -54.7 | NC_006273.1 | + | 204159 | 0.69 | 0.89771 |
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Target: 5'- -uACCGUGUCGCcccgCACGGugGUccGUa -3' miRNA: 3'- uuUGGCGCAGUGa---GUGCCugCAucCA- -5' |
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| 29726 | 3' | -54.7 | NC_006273.1 | + | 209994 | 1.05 | 0.011232 |
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Target: 5'- gAAACCGCGUCACUCACGGACGUAGGUu -3' miRNA: 3'- -UUUGGCGCAGUGAGUGCCUGCAUCCA- -5' |
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| 29726 | 3' | -54.7 | NC_006273.1 | + | 213134 | 0.73 | 0.742243 |
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Target: 5'- gGAGCCGCGUCGCUCGCcGGCGc---- -3' miRNA: 3'- -UUUGGCGCAGUGAGUGcCUGCaucca -5' |
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| 29726 | 3' | -54.7 | NC_006273.1 | + | 234919 | 0.66 | 0.970755 |
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Target: 5'- -uGCCGCGggugugUCGCGGGCGUgugccGGGUg -3' miRNA: 3'- uuUGGCGCagug--AGUGCCUGCA-----UCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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