miRNA: 3'- aGAGUgCCCUUCCGAUCaau -5'
miRNA: 3'- -AGa--GUGCCCUUCCGAUCaau -5'
miRNA: 3'- -AGAGUGCCCUUCCGaUCAau -5'
miRNA: 3'- -AGAGUGCCCUUCCGAUCAAU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29728 | 5' | -41.1 | NC_006273.1 | + | 194505 | 0.73 | 0.999895 |
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Target: 5'- gUUCA-GGGAAGGCUAGa-- -3' miRNA: 3'- aGAGUgCCCUUCCGAUCaau -5' |
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| 29728 | 5' | -41.1 | NC_006273.1 | + | 198758 | 0.77 | 0.995996 |
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Target: 5'- uUCccgCAUGGGAAGGCUGGa-- -3' miRNA: 3'- -AGa--GUGCCCUUCCGAUCaau -5' |
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| 29728 | 5' | -41.1 | NC_006273.1 | + | 100798 | 0.78 | 0.992274 |
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Target: 5'- gUCUUGCGGGAAGGC-GGUg- -3' miRNA: 3'- -AGAGUGCCCUUCCGaUCAau -5' |
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| 29728 | 5' | -41.1 | NC_006273.1 | + | 207427 | 1.04 | 0.142502 |
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Target: 5'- cUCUCACGGGAAGGCUAGUUAg -3' miRNA: 3'- -AGAGUGCCCUUCCGAUCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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