Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2973 | 3' | -58.8 | NC_001493.1 | + | 8352 | 0.68 | 0.638525 |
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Target: 5'- cUCGAUCGcccUGAAgcgcgguuCGGGCCgGGGGGUc- -3' miRNA: 3'- -AGCUGGC---ACUU--------GCCUGGgCCCCCAuu -5' |
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| 2973 | 3' | -58.8 | NC_001493.1 | + | 123906 | 0.68 | 0.638525 |
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Target: 5'- cUCGAUCGcccUGAAgcgcgguuCGGGCCgGGGGGUc- -3' miRNA: 3'- -AGCUGGC---ACUU--------GCCUGGgCCCCCAuu -5' |
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| 2973 | 3' | -58.8 | NC_001493.1 | + | 20754 | 0.69 | 0.588232 |
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Target: 5'- cUGACCGUGAcCGaACCCGGcGGGc-- -3' miRNA: 3'- aGCUGGCACUuGCcUGGGCC-CCCauu -5' |
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| 2973 | 3' | -58.8 | NC_001493.1 | + | 50687 | 0.7 | 0.548507 |
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Target: 5'- gCGGCCGUGggUGGACCUuucuuuuuuguGGuGGUGGu -3' miRNA: 3'- aGCUGGCACuuGCCUGGG-----------CCcCCAUU- -5' |
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| 2973 | 3' | -58.8 | NC_001493.1 | + | 107210 | 0.7 | 0.528952 |
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Target: 5'- gUCGACC-UGAggGCGGcCCCGGuucuGGGUAAa -3' miRNA: 3'- -AGCUGGcACU--UGCCuGGGCC----CCCAUU- -5' |
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| 2973 | 3' | -58.8 | NC_001493.1 | + | 105870 | 0.7 | 0.519271 |
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Target: 5'- aCGACCG-GGuacACGGAgacgagguCCCGGGGGg-- -3' miRNA: 3'- aGCUGGCaCU---UGCCU--------GGGCCCCCauu -5' |
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| 2973 | 3' | -58.8 | NC_001493.1 | + | 107638 | 0.72 | 0.453723 |
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Target: 5'- --uGCCGUGAGuuGAUCCGGGGGUu- -3' miRNA: 3'- agcUGGCACUUgcCUGGGCCCCCAuu -5' |
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| 2973 | 3' | -58.8 | NC_001493.1 | + | 35350 | 0.72 | 0.409759 |
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Target: 5'- aUGACCGUGGACGucguCCCGGGGa--- -3' miRNA: 3'- aGCUGGCACUUGCcu--GGGCCCCcauu -5' |
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| 2973 | 3' | -58.8 | NC_001493.1 | + | 93843 | 1.06 | 0.002197 |
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Target: 5'- aUCGACCGUGAACGGACCCGGGGGUAAa -3' miRNA: 3'- -AGCUGGCACUUGCCUGGGCCCCCAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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