Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
29741 | 5' | -56.7 | NC_006273.1 | + | 92295 | 0.66 | 0.942128 |
Target: 5'- aGGGCUCGCUgaggCUGUaaucgCACAGCGCGg -3' miRNA: 3'- -CUUGAGCGGag--GGCGa----GUGUUGCGCa -5' |
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29741 | 5' | -56.7 | NC_006273.1 | + | 19036 | 0.66 | 0.942128 |
Target: 5'- aGAACUgGgCCUUCCccuCUCACAGCGuCGa -3' miRNA: 3'- -CUUGAgC-GGAGGGc--GAGUGUUGC-GCa -5' |
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29741 | 5' | -56.7 | NC_006273.1 | + | 123008 | 0.66 | 0.946468 |
Target: 5'- cGAGCUCGCaCUgCUgGUUCaACAGCGCc- -3' miRNA: 3'- -CUUGAGCG-GA-GGgCGAG-UGUUGCGca -5' |
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29741 | 5' | -56.7 | NC_006273.1 | + | 187144 | 0.66 | 0.946468 |
Target: 5'- uGGGCUCGCCUCCgUGCggaCGCcguuucgugcaGACGCu- -3' miRNA: 3'- -CUUGAGCGGAGG-GCGa--GUG-----------UUGCGca -5' |
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29741 | 5' | -56.7 | NC_006273.1 | + | 72956 | 0.66 | 0.946468 |
Target: 5'- aGAGCgccgucacCGCCUCCCGCUCggccugccgACAcaccagccauucGCGCa- -3' miRNA: 3'- -CUUGa-------GCGGAGGGCGAG---------UGU------------UGCGca -5' |
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29741 | 5' | -56.7 | NC_006273.1 | + | 203548 | 0.66 | 0.946468 |
Target: 5'- -----gGCgUCCUGCUgacgCACGACGCGUa -3' miRNA: 3'- cuugagCGgAGGGCGA----GUGUUGCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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