Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29741 | 5' | -56.7 | NC_006273.1 | + | 193033 | 0.69 | 0.842798 |
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Target: 5'- aGugUCGCCggaCCUGCUCGuCAGCaGCGa -3' miRNA: 3'- cUugAGCGGa--GGGCGAGU-GUUG-CGCa -5' |
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| 29741 | 5' | -56.7 | NC_006273.1 | + | 197468 | 1.08 | 0.004613 |
|
Target: 5'- cGAACUCGCCUCCCGCUCACAACGCGUg -3' miRNA: 3'- -CUUGAGCGGAGGGCGAGUGUUGCGCA- -5' |
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| 29741 | 5' | -56.7 | NC_006273.1 | + | 198657 | 0.66 | 0.934721 |
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Target: 5'- cGAGCUgugccgccgcucgcgCGCCU-CCGCUCGCGGcCGCu- -3' miRNA: 3'- -CUUGA---------------GCGGAgGGCGAGUGUU-GCGca -5' |
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| 29741 | 5' | -56.7 | NC_006273.1 | + | 199334 | 0.68 | 0.879673 |
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Target: 5'- uGACUCGCuCUCgUCGCUCuauCuGCGCGg -3' miRNA: 3'- cUUGAGCG-GAG-GGCGAGu--GuUGCGCa -5' |
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| 29741 | 5' | -56.7 | NC_006273.1 | + | 203548 | 0.66 | 0.946468 |
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Target: 5'- -----gGCgUCCUGCUgacgCACGACGCGUa -3' miRNA: 3'- cuugagCGgAGGGCGA----GUGUUGCGCA- -5' |
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| 29741 | 5' | -56.7 | NC_006273.1 | + | 219954 | 0.69 | 0.818466 |
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Target: 5'- -cGCUCGCgCUCgcgUCGCUCaucggGCAACGCGg -3' miRNA: 3'- cuUGAGCG-GAG---GGCGAG-----UGUUGCGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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