Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2975 | 3' | -55.3 | NC_001493.1 | + | 24581 | 0.66 | 0.914884 |
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Target: 5'- cUGACCGA-GGAcacguuuuacaaGUGGCUCGcCgAUGAu -3' miRNA: 3'- cACUGGCUaCCU------------CACCGAGCaG-UGCU- -5' |
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| 2975 | 3' | -55.3 | NC_001493.1 | + | 108384 | 0.67 | 0.875289 |
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Target: 5'- -cGACCuGAUGGGcaucCUCGUCACGAg -3' miRNA: 3'- caCUGG-CUACCUcaccGAGCAGUGCU- -5' |
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| 2975 | 3' | -55.3 | NC_001493.1 | + | 119241 | 0.67 | 0.860274 |
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Target: 5'- -cGACCGGucgUGGAGaUGGaCUCGUUugucugugcgGCGAg -3' miRNA: 3'- caCUGGCU---ACCUC-ACC-GAGCAG----------UGCU- -5' |
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| 2975 | 3' | -55.3 | NC_001493.1 | + | 3686 | 0.67 | 0.860274 |
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Target: 5'- -cGACCGGucgUGGAGaUGGaCUCGUUugucugugcgGCGAg -3' miRNA: 3'- caCUGGCU---ACCUC-ACC-GAGCAG----------UGCU- -5' |
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| 2975 | 3' | -55.3 | NC_001493.1 | + | 95049 | 0.68 | 0.810419 |
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Target: 5'- cGUGugCGAUGGGG-GGaUCGUCGgGu -3' miRNA: 3'- -CACugGCUACCUCaCCgAGCAGUgCu -5' |
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| 2975 | 3' | -55.3 | NC_001493.1 | + | 117538 | 0.74 | 0.51141 |
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Target: 5'- uGUGGCCGucaaGGAGUGGCU-GUCuACGGa -3' miRNA: 3'- -CACUGGCua--CCUCACCGAgCAG-UGCU- -5' |
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| 2975 | 3' | -55.3 | NC_001493.1 | + | 1984 | 0.74 | 0.51141 |
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Target: 5'- uGUGGCCGucaaGGAGUGGCU-GUCuACGGa -3' miRNA: 3'- -CACUGGCua--CCUCACCGAgCAG-UGCU- -5' |
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| 2975 | 3' | -55.3 | NC_001493.1 | + | 34931 | 0.77 | 0.33534 |
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Target: 5'- -gGACCGAUGGAucccGUGGUUUGUCACc- -3' miRNA: 3'- caCUGGCUACCU----CACCGAGCAGUGcu -5' |
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| 2975 | 3' | -55.3 | NC_001493.1 | + | 96020 | 1.09 | 0.003169 |
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Target: 5'- uGUGACCGAUGGAGUGGCUCGUCACGAg -3' miRNA: 3'- -CACUGGCUACCUCACCGAGCAGUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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