Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29803 | 3' | -53.4 | NC_006273.1 | + | 192728 | 0.72 | 0.834113 |
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Target: 5'- uCUGg---CUGCUGC-UGCGUGUGUGCu -3' miRNA: 3'- -GACauaaGAUGGCGcGCGCACACGCG- -5' |
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| 29803 | 3' | -53.4 | NC_006273.1 | + | 124430 | 0.73 | 0.817413 |
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Target: 5'- -cGUGcugCgGCCGCGCGgGcGUGCGCg -3' miRNA: 3'- gaCAUaa-GaUGGCGCGCgCaCACGCG- -5' |
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| 29803 | 3' | -53.4 | NC_006273.1 | + | 197242 | 0.75 | 0.705795 |
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Target: 5'- gCUGUcgcacuUUCUGCgCGCGgGCGUGcuggGCGCg -3' miRNA: 3'- -GACAu-----AAGAUG-GCGCgCGCACa---CGCG- -5' |
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| 29803 | 3' | -53.4 | NC_006273.1 | + | 2349 | 0.75 | 0.705795 |
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Target: 5'- gCUGUcgcacuUUCUGCgCGCGgGCGUGcuggGCGCg -3' miRNA: 3'- -GACAu-----AAGAUG-GCGCgCGCACa---CGCG- -5' |
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| 29803 | 3' | -53.4 | NC_006273.1 | + | 176270 | 0.77 | 0.585322 |
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Target: 5'- -cGUGUUaaugaagcgCUACCGUGCGCGUGagcaGCGCu -3' miRNA: 3'- gaCAUAA---------GAUGGCGCGCGCACa---CGCG- -5' |
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| 29803 | 3' | -53.4 | NC_006273.1 | + | 142423 | 1.13 | 0.004782 |
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Target: 5'- gCUGUAUUCUACCGCGCGCGUGUGCGCg -3' miRNA: 3'- -GACAUAAGAUGGCGCGCGCACACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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