Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29803 | 3' | -53.4 | NC_006273.1 | + | 234906 | 0.7 | 0.917661 |
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Target: 5'- gUGUGUcagggUgUGCCGCGgGUGUGU-CGCg -3' miRNA: 3'- gACAUA-----AgAUGGCGCgCGCACAcGCG- -5' |
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| 29803 | 3' | -53.4 | NC_006273.1 | + | 44218 | 0.69 | 0.942963 |
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Target: 5'- aUGggggUUACCGUGauCGCGUcGUGCGCg -3' miRNA: 3'- gACauaaGAUGGCGC--GCGCA-CACGCG- -5' |
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| 29803 | 3' | -53.4 | NC_006273.1 | + | 174105 | 0.69 | 0.947331 |
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Target: 5'- -cGUGUacacggccUCgGCCGCGCGCGcc-GCGCu -3' miRNA: 3'- gaCAUA--------AGaUGGCGCGCGCacaCGCG- -5' |
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| 29803 | 3' | -53.4 | NC_006273.1 | + | 58680 | 0.68 | 0.959086 |
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Target: 5'- uUGUGgcugCUauugggucacaGCCGCGUGCcgcggGUGCGCg -3' miRNA: 3'- gACAUaa--GA-----------UGGCGCGCGca---CACGCG- -5' |
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| 29803 | 3' | -53.4 | NC_006273.1 | + | 43140 | 0.68 | 0.968898 |
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Target: 5'- gUGUGguaUACCGCGUuaggcaccGCGUG-GCGUa -3' miRNA: 3'- gACAUaagAUGGCGCG--------CGCACaCGCG- -5' |
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| 29803 | 3' | -53.4 | NC_006273.1 | + | 142423 | 1.13 | 0.004782 |
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Target: 5'- gCUGUAUUCUACCGCGCGCGUGUGCGCg -3' miRNA: 3'- -GACAUAAGAUGGCGCGCGCACACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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