Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29803 | 5' | -52.8 | NC_006273.1 | + | 93925 | 0.66 | 0.987762 |
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Target: 5'- -gGUGCGCAUGCGCG-GGCcagGACc- -3' miRNA: 3'- caCACGCGUGUGCGCaCUGca-UUGuu -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 4310 | 0.66 | 0.988783 |
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Target: 5'- -gGUGCGCAucacgcucuuguuuCACGCGUugcacagcGACGgugAACAAa -3' miRNA: 3'- caCACGCGU--------------GUGCGCA--------CUGCa--UUGUU- -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 168371 | 0.66 | 0.9892 |
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Target: 5'- -aGU-CGCAgACGCGUcuguucgucgcGACGUAACAu -3' miRNA: 3'- caCAcGCGUgUGCGCA-----------CUGCAUUGUu -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 131478 | 0.66 | 0.9892 |
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Target: 5'- cGUG-GCGUACGC-CGUGGCGc-GCAAc -3' miRNA: 3'- -CACaCGCGUGUGcGCACUGCauUGUU- -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 195526 | 0.66 | 0.990503 |
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Target: 5'- -cGUGCGCGucCACaGCGUGACuuugAGCGg -3' miRNA: 3'- caCACGCGU--GUG-CGCACUGca--UUGUu -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 202794 | 0.66 | 0.99168 |
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Target: 5'- cUGcUGCGuCAC-CGUGUGACGccgGGCAAg -3' miRNA: 3'- cAC-ACGC-GUGuGCGCACUGCa--UUGUU- -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 163600 | 0.66 | 0.99168 |
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Target: 5'- cUGUGCGCGCGCuGUcaGACG-GGCGAg -3' miRNA: 3'- cACACGCGUGUG-CGcaCUGCaUUGUU- -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 210339 | 0.66 | 0.99168 |
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Target: 5'- cGUaUGCGcCACAuCGCGUGgcauaagaACGUGACGGa -3' miRNA: 3'- -CAcACGC-GUGU-GCGCAC--------UGCAUUGUU- -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 32605 | 0.66 | 0.99168 |
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Target: 5'- -gGUGaCGCagcaGCGCGCGgcucACGUAGCAGg -3' miRNA: 3'- caCAC-GCG----UGUGCGCac--UGCAUUGUU- -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 177133 | 0.66 | 0.99168 |
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Target: 5'- gGUG-GCGCAUcUGUGgGGCGUGACGc -3' miRNA: 3'- -CACaCGCGUGuGCGCaCUGCAUUGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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