Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29803 | 5' | -52.8 | NC_006273.1 | + | 83654 | 0.69 | 0.948964 |
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Target: 5'- -cGUGCGCAagGCGCGcGACGcGGCGc -3' miRNA: 3'- caCACGCGUg-UGCGCaCUGCaUUGUu -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 167221 | 0.69 | 0.935139 |
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Target: 5'- ---cGgGUACACGCGUGACGgaGCu- -3' miRNA: 3'- cacaCgCGUGUGCGCACUGCauUGuu -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 216516 | 0.7 | 0.91333 |
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Target: 5'- -aGUGCGCcgugccagcgcaGCACgucucuuuucaGCGUGugGUGACAGa -3' miRNA: 3'- caCACGCG------------UGUG-----------CGCACugCAUUGUU- -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 103384 | 0.7 | 0.91333 |
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Target: 5'- cUGUGCGCAgcgagGCGCauaGACGUAACAAg -3' miRNA: 3'- cACACGCGUg----UGCGca-CUGCAUUGUU- -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 62461 | 0.7 | 0.91333 |
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Target: 5'- -gGUGCcgaaCACAUGCGUGACGUcagcuACAAg -3' miRNA: 3'- caCACGc---GUGUGCGCACUGCAu----UGUU- -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 60898 | 0.72 | 0.842506 |
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Target: 5'- cUG-GCGCGgGCGCGUGAUGcGACGGu -3' miRNA: 3'- cACaCGCGUgUGCGCACUGCaUUGUU- -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 40721 | 0.73 | 0.781151 |
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Target: 5'- uUGUGCGCACugccguUGCGUGGCGUcccGCGGu -3' miRNA: 3'- cACACGCGUGu-----GCGCACUGCAu--UGUU- -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 783 | 0.73 | 0.781151 |
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Target: 5'- uUGUGCGCACugccguUGCGUGGCGUcccGCGGu -3' miRNA: 3'- cACACGCGUGu-----GCGCACUGCAu--UGUU- -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 92941 | 0.74 | 0.752651 |
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Target: 5'- cGUGUGCGCACACGCGcacGCGc-GCAc -3' miRNA: 3'- -CACACGCGUGUGCGCac-UGCauUGUu -5' |
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| 29803 | 5' | -52.8 | NC_006273.1 | + | 142457 | 1.07 | 0.01094 |
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Target: 5'- cGUGUGCGCACACGCGUGACGUAACAAa -3' miRNA: 3'- -CACACGCGUGUGCGCACUGCAUUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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