miRNA: 3'- aUCG---AGCGaa------GCGAGCGau-GCAGUAG- -5'
miRNA: 3'- aUCGAGCGaag------CGAGCGa-----UGCAGuAG- -5'
miRNA: 3'- aUCGAgCGAAGCGA-GCGAugC-AGuag -5'
miRNA: 3'- aucGAGCGaa-----GC-GAGCGAUGCaGUAG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29805 | 3' | -53.7 | NC_006273.1 | + | 34867 | 0.66 | 0.978492 |
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Target: 5'- gAGCggaUCGCgaaauaggCGCUCGUcacCGUCGUCa -3' miRNA: 3'- aUCG---AGCGaa------GCGAGCGau-GCAGUAG- -5' |
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| 29805 | 3' | -53.7 | NC_006273.1 | + | 144452 | 0.66 | 0.980703 |
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Target: 5'- cGGCUCGCacuacacggGCUCGCgcgccgGCGUC-UCg -3' miRNA: 3'- aUCGAGCGaag------CGAGCGa-----UGCAGuAG- -5' |
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| 29805 | 3' | -53.7 | NC_006273.1 | + | 49479 | 0.66 | 0.982739 |
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Target: 5'- aAGCU-GCUUCGCUgCGCUugGaUCc-- -3' miRNA: 3'- aUCGAgCGAAGCGA-GCGAugC-AGuag -5' |
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| 29805 | 3' | -53.7 | NC_006273.1 | + | 135097 | 0.66 | 0.982739 |
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Target: 5'- --uCUCGCgcguggcCGuCUCGCUACGcCGUCg -3' miRNA: 3'- aucGAGCGaa-----GC-GAGCGAUGCaGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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