Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
29805 | 3' | -53.7 | NC_006273.1 | + | 213135 | 0.72 | 0.774413 |
Target: 5'- gAGC-CGCgUCGCUCGCcggcgccCGUCAUCu -3' miRNA: 3'- aUCGaGCGaAGCGAGCGau-----GCAGUAG- -5' |
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29805 | 3' | -53.7 | NC_006273.1 | + | 149514 | 0.75 | 0.635111 |
Target: 5'- -cGCUUGUUgCGCggGCUGCGUCGUCa -3' miRNA: 3'- auCGAGCGAaGCGagCGAUGCAGUAG- -5' |
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29805 | 3' | -53.7 | NC_006273.1 | + | 141940 | 0.92 | 0.072789 |
Target: 5'- gUAGCUCGCUaCGCUCGCUACGUCAc- -3' miRNA: 3'- -AUCGAGCGAaGCGAGCGAUGCAGUag -5' |
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29805 | 3' | -53.7 | NC_006273.1 | + | 141873 | 1.09 | 0.006491 |
Target: 5'- gUAGCUCGCUUCGCUCGCUACGUCAUCa -3' miRNA: 3'- -AUCGAGCGAAGCGAGCGAUGCAGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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