Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29806 | 5' | -53.2 | NC_006273.1 | + | 182781 | 0.73 | 0.788723 |
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Target: 5'- cGAGAAGCCUGcGAGGcucucaaaaaGGCCCugcgGAGGCa -3' miRNA: 3'- uUUUUUCGGGC-CUCC----------CCGGGu---UUUCG- -5' |
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| 29806 | 5' | -53.2 | NC_006273.1 | + | 143949 | 0.73 | 0.750862 |
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Target: 5'- ----uAGCgCCGGuGGGGCCCGc-GGCu -3' miRNA: 3'- uuuuuUCG-GGCCuCCCCGGGUuuUCG- -5' |
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| 29806 | 5' | -53.2 | NC_006273.1 | + | 140164 | 0.76 | 0.61919 |
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Target: 5'- ----cAGCCUGGuGGGGUCC-GAGGCg -3' miRNA: 3'- uuuuuUCGGGCCuCCCCGGGuUUUCG- -5' |
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| 29806 | 5' | -53.2 | NC_006273.1 | + | 156208 | 0.77 | 0.578142 |
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Target: 5'- gAGGAGAGCagcacguuaCCGGAGGGGCUCGAAacaucaucagggGGCu -3' miRNA: 3'- -UUUUUUCG---------GGCCUCCCCGGGUUU------------UCG- -5' |
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| 29806 | 5' | -53.2 | NC_006273.1 | + | 94516 | 0.77 | 0.56796 |
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Target: 5'- ----cGGCCCGGGGGGaCCCGAGcgggGGCu -3' miRNA: 3'- uuuuuUCGGGCCUCCCcGGGUUU----UCG- -5' |
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| 29806 | 5' | -53.2 | NC_006273.1 | + | 140613 | 1.09 | 0.007137 |
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Target: 5'- aAAAAAAGCCCGGAGGGGCCCAAAAGCc -3' miRNA: 3'- -UUUUUUCGGGCCUCCCCGGGUUUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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