Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29807 | 5' | -56.5 | NC_006273.1 | + | 140710 | 0.69 | 0.831756 |
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Target: 5'- uGGuGGUGGAGugGugGCGgcggcGGGGCCgGCg -3' miRNA: 3'- uUC-CCACCUU--UugCGCa----CCUCGGgUG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 26809 | 0.69 | 0.831756 |
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Target: 5'- uGGGGUGGGGAcgggggACGCGguagGGAGCaaaACc -3' miRNA: 3'- uUCCCACCUUU------UGCGCa---CCUCGgg-UG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 199041 | 0.69 | 0.821787 |
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Target: 5'- cAGGGgcuucguGCGCGUGGGGCUgGCg -3' miRNA: 3'- uUCCCaccuuu-UGCGCACCUCGGgUG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 193627 | 0.71 | 0.713183 |
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Target: 5'- gAAGGGUccgucgGGAcAGCGCGUGGGacgugaggccGCUCACg -3' miRNA: 3'- -UUCCCA------CCUuUUGCGCACCU----------CGGGUG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 66056 | 0.71 | 0.69247 |
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Target: 5'- -uGGGUGGAGGagaugcuGCGCuacGUGGAaagcaaGCCCACu -3' miRNA: 3'- uuCCCACCUUU-------UGCG---CACCU------CGGGUG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 117708 | 0.72 | 0.663529 |
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Target: 5'- -cGGGUGGGGucgGCGUuacGAGCCCGCg -3' miRNA: 3'- uuCCCACCUUuugCGCAc--CUCGGGUG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 160322 | 0.72 | 0.643437 |
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Target: 5'- -cGGG-GGAAgaaacGugGUGUGGGGCCCGu -3' miRNA: 3'- uuCCCaCCUU-----UugCGCACCUCGGGUg -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 166149 | 0.72 | 0.62331 |
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Target: 5'- -uGGGcgGGAGGACGCGUgGGAGCgCGg -3' miRNA: 3'- uuCCCa-CCUUUUGCGCA-CCUCGgGUg -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 145578 | 0.73 | 0.583192 |
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Target: 5'- cGGGGGUGGuggacGGGACGUGagcgGGGGCCCGa -3' miRNA: 3'- -UUCCCACC-----UUUUGCGCa---CCUCGGGUg -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 139938 | 1.08 | 0.00421 |
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Target: 5'- aAAGGGUGGAAAACGCGUGGAGCCCACg -3' miRNA: 3'- -UUCCCACCUUUUGCGCACCUCGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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