Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29807 | 5' | -56.5 | NC_006273.1 | + | 189866 | 0.68 | 0.839871 |
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Target: 5'- -cGGG-GGGAGACGCGUcGGGCggaCCGCc -3' miRNA: 3'- uuCCCaCCUUUUGCGCAcCUCG---GGUG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 99063 | 0.68 | 0.863094 |
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Target: 5'- -uGGGaGGGAGAgGaCGUGGGGCUCGa -3' miRNA: 3'- uuCCCaCCUUUUgC-GCACCUCGGGUg -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 112227 | 0.67 | 0.884507 |
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Target: 5'- cGGcGGUGGcAAuGCGCGgcgGGGGCUgGCg -3' miRNA: 3'- uUC-CCACC-UUuUGCGCa--CCUCGGgUG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 142300 | 0.67 | 0.887217 |
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Target: 5'- -cGGcGUGGAcgcggugcucgaagaAAACGaCGUGGAGCUgCGCg -3' miRNA: 3'- uuCC-CACCU---------------UUUGC-GCACCUCGG-GUG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 196844 | 0.67 | 0.91002 |
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Target: 5'- cGGuGGcUGGGuuGCGCGgcGGGGCCgGCg -3' miRNA: 3'- uUC-CC-ACCUuuUGCGCa-CCUCGGgUG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 104488 | 0.66 | 0.915834 |
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Target: 5'- cAGGG-GGAAGACGCaGUGGuccgucgguGUCUGCg -3' miRNA: 3'- uUCCCaCCUUUUGCG-CACCu--------CGGGUG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 216866 | 0.66 | 0.92677 |
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Target: 5'- aAAGGccGGcAGGCGCGcaggcccucgaUGGAGCCCAg -3' miRNA: 3'- -UUCCcaCCuUUUGCGC-----------ACCUCGGGUg -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 84868 | 0.66 | 0.931892 |
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Target: 5'- cAGGGUGGAGGGCuuGgccacgGGAGCUguuggCACg -3' miRNA: 3'- uUCCCACCUUUUGcgCa-----CCUCGG-----GUG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 192299 | 0.66 | 0.941443 |
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Target: 5'- -cGuGUGGAAAACGCGUugcgucgcaGAGUCCGCc -3' miRNA: 3'- uuCcCACCUUUUGCGCAc--------CUCGGGUG- -5' |
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| 29807 | 5' | -56.5 | NC_006273.1 | + | 166149 | 0.72 | 0.62331 |
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Target: 5'- -uGGGcgGGAGGACGCGUgGGAGCgCGg -3' miRNA: 3'- uuCCCa-CCUUUUGCGCA-CCUCGgGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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