Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29808 | 5' | -54.9 | NC_006273.1 | + | 2219 | 0.72 | 0.744721 |
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Target: 5'- cGACGGCgAUGGCGgguccggcGGCGucGGGGACCGUg -3' miRNA: 3'- -UUGCCG-UACUGC--------UCGCu-CUCUUGGCA- -5' |
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| 29808 | 5' | -54.9 | NC_006273.1 | + | 29828 | 0.75 | 0.595353 |
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Target: 5'- uGGCGGCAgccacgGAgGAGaCGAGAGAACCu- -3' miRNA: 3'- -UUGCCGUa-----CUgCUC-GCUCUCUUGGca -5' |
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| 29808 | 5' | -54.9 | NC_006273.1 | + | 136881 | 0.76 | 0.555454 |
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Target: 5'- --aGGCAcGACGAGCGAGGGccCCGUc -3' miRNA: 3'- uugCCGUaCUGCUCGCUCUCuuGGCA- -5' |
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| 29808 | 5' | -54.9 | NC_006273.1 | + | 189856 | 0.76 | 0.549531 |
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Target: 5'- uGCGGCAUGGCGGG-GGGAGAcgcgucgggcggaccGCCGg -3' miRNA: 3'- uUGCCGUACUGCUCgCUCUCU---------------UGGCa -5' |
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| 29808 | 5' | -54.9 | NC_006273.1 | + | 118888 | 0.77 | 0.4878 |
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Target: 5'- cGAgGGgAUGACGAGCGuGAGAGCCc- -3' miRNA: 3'- -UUgCCgUACUGCUCGCuCUCUUGGca -5' |
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| 29808 | 5' | -54.9 | NC_006273.1 | + | 139564 | 1.05 | 0.010281 |
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Target: 5'- gAACGGCAUGACGAGCGAGAGAACCGUc -3' miRNA: 3'- -UUGCCGUACUGCUCGCUCUCUUGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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