Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2981 | 3' | -52.9 | NC_001493.1 | + | 45248 | 0.69 | 0.883589 |
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Target: 5'- cCGGccgCGAUGUU--GGUgGCUGCCCGu -3' miRNA: 3'- uGCUa--GCUACAAacCCAgUGACGGGC- -5' |
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| 2981 | 3' | -52.9 | NC_001493.1 | + | 34259 | 0.69 | 0.876326 |
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Target: 5'- gUGAUCGAgcacgUGggUGGGUCcgccaGCUGCCgGu -3' miRNA: 3'- uGCUAGCU-----ACaaACCCAG-----UGACGGgC- -5' |
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| 2981 | 3' | -52.9 | NC_001493.1 | + | 81240 | 0.7 | 0.828149 |
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Target: 5'- gGCGAUCGAggagGUcgUGGGUC--UGCUCGu -3' miRNA: 3'- -UGCUAGCUa---CAa-ACCCAGugACGGGC- -5' |
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| 2981 | 3' | -52.9 | NC_001493.1 | + | 1798 | 0.71 | 0.810507 |
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Target: 5'- uCGAUCGAcc-UUGGGUCACcGCCUu -3' miRNA: 3'- uGCUAGCUacaAACCCAGUGaCGGGc -5' |
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| 2981 | 3' | -52.9 | NC_001493.1 | + | 117352 | 0.71 | 0.810507 |
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Target: 5'- uCGAUCGAcc-UUGGGUCACcGCCUu -3' miRNA: 3'- uGCUAGCUacaAACCCAGUGaCGGGc -5' |
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| 2981 | 3' | -52.9 | NC_001493.1 | + | 99724 | 1.1 | 0.004638 |
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Target: 5'- cACGAUCGAUGUUUGGGUCACUGCCCGg -3' miRNA: 3'- -UGCUAGCUACAAACCCAGUGACGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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