Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29852 | 5' | -56.4 | NC_006273.1 | + | 38315 | 0.66 | 0.948532 |
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Target: 5'- cCUGUUGAcCGUCGCCGUgucgUCGCcg-CCa -3' miRNA: 3'- -GACAGCU-GCGGCGGCG----AGUGaaaGGa -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 93178 | 0.66 | 0.948532 |
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Target: 5'- -cGUCcaccGCGgaCGCCGCUCACgcugUCCg -3' miRNA: 3'- gaCAGc---UGCg-GCGGCGAGUGaa--AGGa -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 177554 | 0.66 | 0.952536 |
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Target: 5'- uUGUUGGUGCCGCCGCg-ACggUUCUg -3' miRNA: 3'- gACAGCUGCGGCGGCGagUGaaAGGA- -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 89364 | 0.66 | 0.952536 |
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Target: 5'- gUGUCGG-GCCGCgGCagUCGCUUagCCa -3' miRNA: 3'- gACAGCUgCGGCGgCG--AGUGAAa-GGa -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 201964 | 0.66 | 0.955211 |
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Target: 5'- gCUGUCGcGCGCCGCCGacgcccgaggcggugCGCg-UCCa -3' miRNA: 3'- -GACAGC-UGCGGCGGCga-------------GUGaaAGGa -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 151789 | 0.66 | 0.956325 |
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Target: 5'- -cGUCG-CGCgCGCCcagGCUCAUggagUCCUc -3' miRNA: 3'- gaCAGCuGCG-GCGG---CGAGUGaa--AGGA- -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 139870 | 0.66 | 0.956325 |
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Target: 5'- gCUGUCGGCgcagucuccgGCCGCCGauuuCUCGgUcUCCg -3' miRNA: 3'- -GACAGCUG----------CGGCGGC----GAGUgAaAGGa -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 173356 | 0.66 | 0.956325 |
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Target: 5'- -cG-CGGUGCCGCCGCUCugUUcggCUa -3' miRNA: 3'- gaCaGCUGCGGCGGCGAGugAAa--GGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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