Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29852 | 5' | -56.4 | NC_006273.1 | + | 128756 | 0.69 | 0.854581 |
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Target: 5'- -cGUCGccauuacCGUCGCCGCUCAaagUUCCc -3' miRNA: 3'- gaCAGCu------GCGGCGGCGAGUga-AAGGa -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 1851 | 0.69 | 0.846936 |
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Target: 5'- -cGUCGGCGCCGCacccCGCgUCGCUgcugacggCCg -3' miRNA: 3'- gaCAGCUGCGGCG----GCG-AGUGAaa------GGa -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 170114 | 0.69 | 0.839109 |
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Target: 5'- -gGUCGuuguCGCUGCCGUguccgCGCUUUCUc -3' miRNA: 3'- gaCAGCu---GCGGCGGCGa----GUGAAAGGa -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 68065 | 0.7 | 0.822931 |
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Target: 5'- -cGcCGugGCCGCCGCUgccgcCGCUguggCCg -3' miRNA: 3'- gaCaGCugCGGCGGCGA-----GUGAaa--GGa -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 88848 | 0.7 | 0.806103 |
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Target: 5'- gUGUCG-CGCCGuCCGCU-ACUUUCg- -3' miRNA: 3'- gACAGCuGCGGC-GGCGAgUGAAAGga -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 121650 | 0.7 | 0.797464 |
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Target: 5'- ---aCGGCGCCGCCGCcgUCGCcaccUCCg -3' miRNA: 3'- gacaGCUGCGGCGGCG--AGUGaa--AGGa -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 171695 | 0.7 | 0.779773 |
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Target: 5'- aCUGcCGGCGCUGCUGCgcaUCACg-UCCUc -3' miRNA: 3'- -GACaGCUGCGGCGGCG---AGUGaaAGGA- -5' |
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| 29852 | 5' | -56.4 | NC_006273.1 | + | 111284 | 1.07 | 0.006035 |
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Target: 5'- uCUGUCGACGCCGCCGCUCACUUUCCUc -3' miRNA: 3'- -GACAGCUGCGGCGGCGAGUGAAAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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